ETI signaling nodes are involved in resistance of Hawaii 7996 to Ralstonia solanacearum-induced bacterial wilt disease in tomato.
ETI signaling nodes are involved in resistance of Hawaii 7996 to Ralstonia solanacearum-induced bacterial wilt disease in tomato.
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DOI：
10.1080/15592324.2023.2194747
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发表时间：
2023-12-31
影响因子：
2.9
通讯作者：
Li, Bo
中科院分区：
文献类型：
--
作者：
Xu, Ai;Wei, Lan;Ke, Jingjing;Peng, Chengfeng;Li, Pengyue;Fan, Changqiu;Yu, Xiao;Li, Bo
青枯病是由土传病原菌青枯雷尔氏菌（Ralstonia solanacearum）引起的番茄毁灭性病害。番茄品种夏威夷7996对番茄霜霉病具有稳定的抗性。青枯菌属但夏威夷7996的抗性机制尚未揭示。在此，我们发现夏威夷7996激活了根细胞死亡反应，并表现出比感病品种Moneymaker更强的防御基因诱导。青枯菌GMI 1000感染。通过采用病毒诱导的基因沉默（VIGS）和CRISPR/Cas9技术，我们发现SlNRG 1沉默和SlADR 1沉默/敲除突变体番茄对青枯病的抗性部分或完全丧失，表明辅助NLR SlADR 1和SlNRG 1是夏威夷7996抗性所必需的，是效应子触发免疫（ETI）途径的关键节点。另外，S1 NDR 1对夏威夷7996的抗病性也有一定的影响。青枯菌、SlEDS 1、SlSAG 101 a/B和SlPAD 4是夏威夷7996中免疫信号传导途径所必需的。总体而言，我们的研究结果表明，夏威夷7996对R.青枯菌依赖于ETI信号通路的多个保守关键节点的参与。本研究揭示了番茄抗病的分子机制。并将加速培育番茄抗病能力。
Bacterial wilt caused by the soil-borne pathogen Ralstonia solanacearum is a destructive disease of tomato. Tomato cultivar Hawaii 7996 is well-known for its stable resistance against R. solanacearum. However, the resistance mechanism of Hawaii 7996 has not yet been revealed. Here, we showed that Hawaii 7996 activated root cell death response and exhibited stronger defense gene induction than the susceptible cultivar Moneymaker after R. solanacearum GMI1000 infection. By employing virus-induced gene silencing (VIGS) and CRISPR/Cas9 technologies, we found that SlNRG1-silenced and SlADR1-silenced/knockout mutant tomato partially or completely lost resistance to bacterial wilt, indicating that helper NLRs SlADR1 and SlNRG1, the key nodes of effector-triggered immunity (ETI) pathways, are required for Hawaii 7996 resistance. In addition, while SlNDR1 was dispensable for the resistance of Hawaii 7996 to R. solanacearum, SlEDS1, SlSAG101a/b, and SlPAD4 were essential for the immune signaling pathways in Hawaii 7996. Overall, our results suggested that robust resistance of Hawaii 7996 to R. solanacearum relied on the involvement of multiple conserved key nodes of the ETI signaling pathways. This study sheds light on the molecular mechanisms underlying tomato resistance to R. solanacearum and will accelerate the breeding of tomatoes resilient to diseases.
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影响因子：
11.6
作者：
Lapin, Dmitry;Kovacova, Viera;Parker, Jane E.
通讯作者：
Parker, Jane E.
影响因子：
7.2
作者：
Catanzariti, Ann-Maree;Do, Huong T. T.;Jones, David A.
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Jones, David A.
影响因子：
--
作者：
Kim, Nayoung;Kang, Won-Hee;Yeom, Seon-In
通讯作者：
Yeom, Seon-In
DOI：
10.1073/pnas.92.14.6597
发表时间：
1995-07-03
影响因子：
11.1
作者：
CENTURY, KS;HOLUB, EB;STASKAWICZ, BJ
通讯作者：
STASKAWICZ, BJ
影响因子：
3.2
作者：
McGarvey, JA;Denny, TP;Schell, MA
通讯作者：
Schell, MA