Insight Into Metabolic Versatility of an Aromatic Compounds-Degrading Arthrobacter sp. YC-RL1.
Insight Into Metabolic Versatility of an Aromatic Compounds-Degrading Arthrobacter sp. YC-RL1.
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深入了解芳香化合物降解节杆菌 YC-RL1 的代谢多样性
DOI：
10.3389/fmicb.2018.02438
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发表时间：
2018
影响因子：
5.2
通讯作者：
Yan Y
中科院分区：
文献类型：
--
作者：
Ren L;Jia Y;Zhang R;Lin Z;Zhen Z;Hu H;Yan Y
节肢杆菌属广泛分布于不同的自然环境中。许多外源性降解节杆菌菌株已被分离和描述；然而，很少有人系统地描述了多种相互关联的代谢途径和编码它们的基因。本研究考察了7种芳香族化合物在YC-RL1节肢菌中的生物降解性。菌株YC-RL1在分离和混合条件下均能高效降解对二甲苯（PX）、萘、菲、联苯、对硝基苯酚（PNP）和双酚A （BPA）。根据检测到的代谢中间体，提出了菌株YC-RL1对萘、联苯、PNP和BPA的代谢途径，表明菌株YC-RL1对芳香族化合物具有系统的代谢途径。此外，基因组分析揭示了部分基因参与提出的途径。在菌株YC-RL1的基因组中分别鉴定出醇内和醇外环切割双加氧酶基因。同时，鉴定出了降解联苯（bph）、对取代酚（npd）和原儿茶酸（pca）的基因簇，并提出了bphA1A2BCD为新的联苯降解基因簇。通过中间体和功能基因分析（bph和pca基因簇）推断了联苯的完整代谢途径。在bph基因簇中，克隆了一个预测的2,3-二羟基联苯1,2-双加氧酶（BphC）基因，并通过异源表达证实了其活性。本工作通过代谢产物鉴定、基因组学分析和实验室实验相结合，系统地阐明了YC-RL1菌株芳香族化合物的代谢多样性。这些结果表明，菌株YC-RL1可能是芳香族化合物污染场地生物修复的一个有希望的候选菌株。
The genus Arthrobacter is ubiquitously distributed in different natural environments. Many xenobiotic-degrading Arthrobacter strains have been isolated and described; however, few have been systematically characterized with regard to multiple interrelated metabolic pathways and the genes that encode them. In this study, the biodegradability of seven aromatic compounds by Arthrobacter sp. YC-RL1 was investigated. Strain YC-RL1 could efficiently degrade p-xylene (PX), naphthalene, phenanthrene, biphenyl, p-nitrophenol (PNP), and bisphenol A (BPA) under both separated and mixed conditions. Based on the detected metabolic intermediates, metabolic pathways of naphthalene, biphenyl, PNP, and BPA were proposed, which indicated that strain YC-RL1 harbors systematic metabolic pathways toward aromatic compounds. Further, genomic analysis uncovered part of genes involved in the proposed pathways. Both intradiol and extradiol ring-cleavage dioxygenase genes were identified in the genome of strain YC-RL1. Meanwhile, gene clusters predicted to encode the degradation of biphenyl (bph), para-substituted phenols (npd) and protocatechuate (pca) were identified, and bphA1A2BCD was proposed to be a novel biphenyl-degrading gene cluster. The complete metabolic pathway of biphenyl was deduced via intermediates and functional gene analysis (bph and pca gene clusters). One of the these genes encoding ring-cleavage dioxygenase in bph gene cluster, a predicted 2,3-dihydroxybiphenyl 1,2-dioxygenase (BphC) gene, was cloned and its activity was confirmed by heterologous expression. This work systematically illuminated the metabolic versatility of aromatic compounds in strain YC-RL1 via the combination of metabolites identification, genomics analysis and laboratory experiments. These results suggested that strain YC-RL1 might be a promising candidate for the bioremediation of aromatic compounds pollution sites.
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影响因子：
11.4
作者：
Boxall, ABA;Sinclair, CJ;Maud, SJ
通讯作者：
Maud, SJ
DOI：
10.1006/bbrc.2000.2500
发表时间：
2000-04-21
影响因子：
3.1
作者：
Chauhan, A;Chakraborti, AK;Jain, RK
通讯作者：
Jain, RK
影响因子：
3.2
作者：
KIKUCHI, Y;YASUKOCHI, Y;TAKAGI, M
通讯作者：
TAKAGI, M
影响因子：
5.2
作者：
Gran-Scheuch A;Fuentes E;Bravo DM;Jiménez JC;Pérez-Donoso JM
通讯作者：
Pérez-Donoso JM
影响因子：
4.4
作者：
Hauschild, JE;Masai, E;Yano, K
通讯作者：
Yano, K