Differential Phototactic Behavior of Closely Related Cyanobacterial Isolates from Yellowstone Hot Spring Biofilms
Differential Phototactic Behavior of Closely Related Cyanobacterial Isolates from Yellowstone Hot Spring Biofilms
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黄石温泉生物膜中密切相关的蓝藻分离株的差异趋光行为
DOI：
10.1128/aem.00196-22
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发表时间：
2022
影响因子：
4.4
通讯作者：
Bhaya, Devaki
中科院分区：
文献类型：
--
作者：
Bunbury, Freddy;Rivas, Carlos;Calatrava, Victoria;Shelton, Amanda N.;Grossman, Arthur;Bhaya, Devaki
在大多数环境中，光养生物膜在整个昼夜循环中经历光照水平的重大变化。趋光性可以是一个有用的策略，优化在这些条件下的曝光，但鲜为人知的是，它的作用，从温泉蓝藻。我们研究了黄石国家公园章鱼泉流出物中两种密切相关的聚球藻（聚球藻OS-A在60至65°C占优势，OS-B′在50至55°C占优势）。两株菌在白色光下均表现出趋光性和趋光性，但在速度和运动性方面存在差异。OS-B′对UVA、蓝光、绿色和红光具有趋光性，而OS-A主要对红光和绿色具有趋光性。在某些条件下，OS-A也表现出负趋光性。在这两个分离株的光感受器和信号转导元件的剧目是很大的不同，其特征在于在其他单细胞蓝藻。OS-A和OS-B′之间光响应的这些差异结合原位观测表明趋光策略可能是非常通用的，并且可以根据光和局部环境进行微调。重要性优化光吸收对光合生物至关重要。一些光合微生物已经进化出一种称为趋光性的复杂过程，以接近或远离光源。在黄石国家公园的许多温泉中，蓝细菌在厚厚的、层状的生物膜或微生物垫中茁壮成长，在那里，微小的运动会导致光照的巨大变化。我们量化的光依赖的运动行为，代表两个最丰富的和密切相关的蓝藻物种从这些弹簧分离。我们发现，它们在速度、方向性以及对不同强度或质量的光的反应方面表现出意想不到的差异。对它们的基因组进行的检查显示，它们的趋光性相关基因有几个变异。研究这些最近分离的蓝藻揭示了不同的趋光策略，甚至可以存在于同一环境中的近亲之间。它还提供了对趋光性在微生物生物膜群落中生长和存活的重要性的见解。
Phototrophic biofilms in most environments experience major changes in light levels throughout a diel cycle. Phototaxis can be a useful strategy for optimizing light exposure under these conditions, but little is known about its role in cyanobacteria from thermal springs. We examined two closely relatedSynechococcusisolates (SynechococcusOS-A dominates at 60 to 65°C and OS-B′ at 50 to 55°C) from outflows of Octopus Spring in Yellowstone National Park. Both isolates exhibited phototaxis and photokinesis in white light, but with differences in speed and motility bias. OS-B′ exhibited phototaxis toward UVA, blue, green, and red wavelengths, while OS-A primarily exhibited phototaxis toward red and green. OS-A also exhibited negative phototaxis under certain conditions. The repertoires of photoreceptors and signal transduction elements in both isolates were quite different from those characterized in other unicellular cyanobacteria. These differences in the photoresponses between OS-A and OS-B′ in conjunction within situobservations indicate that phototactic strategies may be quite versatile and finely tuned to the light and local environment.IMPORTANCEOptimizing light absorption is of paramount importance to photosynthetic organisms. Some photosynthetic microbes have evolved a sophisticated process called phototaxis to move toward or away from a light source. In many hot springs in Yellowstone National Park, cyanobacteria thrive in thick, laminated biofilms or microbial mats, where small movements can result in large changes in light exposure. We quantified the light-dependent motility behaviors in isolates representing two of the most abundant and closely related cyanobacterial species from these springs. We found that they exhibited unexpected differences in their speed, directionality, and responses to different intensities or qualities of light. An examination of their genomes revealed several variations from well-studied phototaxis-related genes. Studying these recently isolated cyanobacteria reveals that diverse phototactic strategies can exist even among close relatives in the same environment. It also provides insights into the importance of phototaxis for growth and survival in microbial biofilm communities.
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影响因子：
11.3
作者：
Wilde A;Mullineaux CW
通讯作者：
Mullineaux CW
三个关键残基对 D 环的不寻常固定促进了没有第二个 Cys 的 DXCF 型蓝藻色素中藻紫胆素的形成。
DOI：
10.1042/bcj20210013
发表时间：
2021
期刊：
The Biochemical journal
影响因子：
--
作者：
K. Fushimi;R. Narikawa
通讯作者：
R. Narikawa
影响因子：
5.4
作者：
Yu Y;You L;Liu D;Hollinshead W;Tang YJ;Zhang F
通讯作者：
Zhang F
影响因子：
12.9
作者：
D. M. Ward;M. Ferris;S. Nold;M. Bateson
通讯作者：
D. M. Ward;M. Ferris;S. Nold;M. Bateson
DOI：
10.1073/pnas.1812871115
发表时间：
2018-12
期刊：
Proceedings of the National Academy of Sciences
影响因子：
--
作者：
Yiling Yang;Vinson Lam;M. Adomako;Ryan Simkovsky;A. Jakob;N. Rockwell;Susan E. Cohen;Susan E. Cohen;A. Taton;Jingtong Wang;J. Lagarias;A. Wilde;David Nobles;J. Brand;S. Golden
通讯作者：
Yiling Yang;Vinson Lam;M. Adomako;Ryan Simkovsky;A. Jakob;N. Rockwell;Susan E. Cohen;Susan E. Cohen;A. Taton;Jingtong Wang;J. Lagarias;A. Wilde;David Nobles;J. Brand;S. Golden