A modular microfluidic device that uses magnetically actuatable microposts for enhanced magnetic bead-based workflows.
A modular microfluidic device that uses magnetically actuatable microposts for enhanced magnetic bead-based workflows.
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DOI：
10.1039/d2lc00859a
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发表时间：
2023-01-17
期刊：
影响因子：
6.1
通讯作者：
中科院分区：
文献类型：
--
作者：
磁珠已经广泛且成功地用于生命科学测定中的靶富集。存在大量可商购获得的功能化用于特异性靶捕获的磁珠，以及能够针对定制应用进行简单表面修饰的选项。虽然磁珠非常适合用于典型实验室工作流程的宏观流体环境中，但它们的性能在微流体环境中会下降，例如用于即时诊断的耗材。一个主要原因是在这些低雷诺数环境中的扩散限制的分析物运输。一种新的方法，BeadPak，使用磁致动微柱，以加强分析物的运输，提高所需的目标产量。定义了该技术操作的关键参数，并在典型生命科学分析中表征了其性能。BeadPak的捕获速度比仅依靠扩散的微流控室快1000倍，通过体积减少实现了显著的样本浓缩，并证明了与一系列生物样本的兼容性。在这项工作中显示的结果可以扩展到其他系统，利用磁珠的目标捕获，浓缩，和/或纯化。
Magnetic beads have been widely and successfully used for target enrichment in life science assays. There exists a large variety of commercially available magnetic beads functionalized for specific target capture, as well as options that enable simple surface modifications for custom applications. While magnetic beads are ideal for use in the macrofluidic context of typical laboratory workflows, their performance drops in microfluidic contexts, such as consumables for point-of-care diagnostics. A primary cause is the diffusion-limited analyte transport in these low Reynolds number environments. A new method, BeadPak, uses magnetically actuatable microposts to enhance analyte transport, improving yield of the desired targets. Critical parameters were defined for the operation of this technology and its performance characterized in canonical life-science assays. BeadPak achieved up to 1000× faster capture than a microfluidic chamber relying on diffusion alone, enabled a significant specimen concentration via volume reduction, and demonstrated compatibility with a range of biological specimens. The results shown in this work can be extended to other systems that utilize magnetic beads for target capture, concentration, and/or purification.
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影响因子：
5.2
作者：
McAnena P;Brown JA;Kerin MJ
通讯作者：
Kerin MJ
影响因子：
9.4
作者：
Cabibbe AM;Spitaleri A;Battaglia S;Colman RE;Suresh A;Uplekar S;Rodwell TC;Cirillo DM
通讯作者：
Cirillo DM
影响因子：
5.7
作者：
Rahier JF;Druez A;Faugeras L;Martinet JP;Géhénot M;Josseaux E;Herzog M;Micallef J;George F;Delos M;De Ronde T;Badaoui A;D'Hondt L
通讯作者：
D'Hondt L
影响因子：
3.8
作者：
Müller K;Daßen S;Holowachuk S;Zwirglmaier K;Stehr J;Buersgens F;Ullerich L;Stoecker K
通讯作者：
Stoecker K
影响因子：
--
作者：
Ali N;Rampazzo RCP;Costa ADT;Krieger MA
通讯作者：
Krieger MA