Retro-orbital injections in mice.

Retro-orbital injections in mice.
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DOI:
10.1038/laban0511-155
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发表时间:
2011-05
期刊:
影响因子:
6.9
通讯作者:
--
中科院分区:
农林科学3区
文献类型:
--
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静脉内血管通路在成年小鼠中具有技术挑战性,在新生小鼠中更具挑战性。作者描述了成年和新生小鼠的静脉窦眶后注射技术。该技术是尾静脉注射给药非致瘤化合物的有用替代方法。作者报告称,他们已在成年小鼠中常规使用该技术,给药体积高达150 µl,未发生任何事件。眶后注射给药在新生小鼠中更具挑战性,但可以可靠地输送高达10 µl的体积。
Intravenous vascular access is technically challenging in the adult mouse and even more challenging in neonatal mice. The authors describe the technique of retro-orbital injection of the venous sinus in the adult and neonatal mouse. This technique is a useful alternative to tail vein injection for the administration of non-tumorigenic compounds. The authors report that they have routinely used this technique in the adult mouse to administer volumes up to 150 µl without incident. Administration of retro-orbital injections is more challenging in neonatal mice but can reliably deliver volumes up to 10 µl.
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