Molecular Cloning and Functional Analysis of the NPR1 Homolog in Kiwifruit (Actinidia eriantha).
Molecular Cloning and Functional Analysis of the NPR1 Homolog in Kiwifruit (Actinidia eriantha).
复制标题
猕猴桃 (Actinidia eriantha) NPR1 同源物的分子克隆和功能分析
DOI：
10.3389/fpls.2020.551201
复制
发表时间：
2020
影响因子：
5.6
通讯作者：
Chen JY
中科院分区：
文献类型：
--
作者：
Sun LM;Fang JB;Zhang M;Qi XJ;Lin MM;Chen JY
猕猴桃细菌性溃烂病是由细菌病原菌丁香假单胞菌引起的。猕猴桃腐烂病(Acinidiae，PSA)是世界范围内猕猴桃生产中的一种破坏性病害。因此，了解猕猴桃对病原菌的防御机制有助于开发有效的新型保护策略。病程相关基因非表达基因1(NPR1)是水杨酸(SA)依赖的信号通路的重要组成部分。本研究利用聚合酶链式反应和快速扩增技术分离到一个新的猕猴桃类NPR1基因，命名为AeNPR1a。该基因全长为1952个碱基对，1,746个碱基对，编码582个氨基酸。同源性分析表明，AeNPR1a蛋白与葡萄的VvNPR1蛋白有显著的相似性。克隆了AeNPR1a基因的一个2.0kb的5‘侧翼区，序列分析表明该基因含有几个可能的顺式调控元件，包括基本元件、防御和胁迫反应元件以及WRKY转录因子的结合部位。实时荧光定量聚合酶链式反应结果表明，AeNPR1a在不同组织中的表达模式不同，植物激素处理和PSA接种均可诱导其转录。酵母双杂交实验表明AeNPR1a与AeTGA2相互作用。AeNPR1a的组成性表达诱导了转基因烟草中致病相关基因的表达，提高了烟草对细菌病原菌的耐受性。此外，AeNPR1a的表达还可以恢复对丁香假单胞菌的基础抗性。拟南芥npr1-1突变体中的番茄DC3000(PST)。我们的数据表明，AeNPR1a基因可能在猕猴桃的防御反应中发挥关键作用。
Kiwifruit bacterial canker, caused by the bacterial pathogen Pseudomonas syringae pv. actinidiae (Psa), is a destructive disease in the kiwifruit industry globally. Consequently, understanding the mechanism of defense against pathogens in kiwifruit could facilitate the development of effective novel protection strategies. The Non-expressor of Pathogenesis-Related genes 1 (NPR1) is a critical component of the salicylic acid (SA)-dependent signaling pathway. Here, a novel kiwifruit NPR1-like gene, designated AeNPR1a, was isolated by using PCR and rapid amplification of cDNA ends techniques. The full-length cDNA consisted of 1952 base pairs with a 1,746-bp open-reading frame encoding a 582 amino acid protein. Homology analysis showed that the AeNPR1a protein is significantly similar to the VvNPR1 of grape. A 2.0 Kb 5′-flanking region of AeNPR1a was isolated, and sequence identification revealed the presence of several putative cis-regulatory elements, including basic elements, defense and stress response elements, and binding sites for WRKY transcription factors. Real-time quantitative PCR results demonstrated that AeNPR1a had different expression patterns in various tissues, and its transcription could be induced by phytohormone treatment and Psa inoculation. The yeast two-hybrid assay revealed that AeNPR1a interacts with AeTGA2. Constitutive expression of AeNPR1a induced the expression of pathogenesis-related gene in transgenic tobacco plants and enhanced tolerance to bacterial pathogens. In addition, AeNPR1a expression could restore basal resistance to Pseudomonas syringae pv. tomato DC3000 (Pst) in Arabidopsis npr1-1 mutant. Our data suggest that AeNPR1a gene is likely to play a pivotal role in defense responses in kiwifruit.
登录
查看更多内容
影响因子：
3.5
作者：
Friedrich, L;Lawton, K;Ryals, J
通讯作者：
Ryals, J
影响因子：
11.6
作者：
CAO, H;BOWLING, SA;DONG, XN
通讯作者：
DONG, XN
全身性获得阻力的信号调节剂。
影响因子：
5.6
作者：
Gao QM;Zhu S;Kachroo P;Kachroo A
通讯作者：
Kachroo A
影响因子：
7.2
作者：
Clough, SJ;Bent, AF
通讯作者：
Bent, AF
影响因子：
2.6
作者：
Japelaghi, Reza Heidari;Haddad, Raheem;Garoosi, Ghasem-Ali
通讯作者：
Garoosi, Ghasem-Ali