Mueller matrix imaging for collagen scoring in mice model of pregnancy.
Mueller matrix imaging for collagen scoring in mice model of pregnancy.
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DOI：
10.1038/s41598-021-95020-8
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发表时间：
2021-08-02
影响因子：
4.6
通讯作者：
Novikova T
中科院分区：
文献类型：
--
作者：
Lee HR;Saytashev I;Du Le VN;Mahendroo M;Ramella-Roman J;Novikova T
早产风险与妊娠子宫颈的早期软化有关，这是由于胶原蛋白细胞外基质的加速重塑而导致的。对妊娠模型的研究在不同时间的妊娠点使用成像木旋偏光仪进行了研究，以找到用于胶原蛋白评分的偏振参数。在19天妊娠期的第6天和第18天和通过宫颈的不同空间位置拍摄了小鼠子宫内宫颈的未染色切片的Mueller矩阵图像。记录的Mueller矩阵的对数分解映射了颈组织光学轴的去极化，线性延迟和方位角。这些图像强调了子宫颈的内部结构和第二次谐波生成显微镜证实的宫颈胶原纤维的排列。在整个宫颈组织图像中线性延迟和线性去极化的分布的统计分析和两高斯的拟合度（无需手动选择特定的感兴趣区域）量化了胶原蛋白纤维与妊娠时间对齐的随机化。在第18天，可以在体内直接光学观测的外部宫颈OS上测量胶原蛋白的宫颈细胞外基质的重塑。它支持这样的假设，即成像穆勒极光学有望在怀孕中快速准确的胶原蛋白评分以及对早产的评估。
Preterm birth risk is associated with early softening of the uterine cervix in pregnancy due to the accelerated remodeling of collagen extracellular matrix. Studies of mice model of pregnancy were performed with an imaging Mueller polarimeter at different time points of pregnancy to find polarimetric parameters for collagen scoring. Mueller matrix images of the unstained sections of mice uterine cervices were taken at day 6 and day 18 of 19-days gestation period and at different spatial locations through the cervices. The logarithmic decomposition of the recorded Mueller matrices mapped the depolarization, linear retardance, and azimuth of the optical axis of cervical tissue. These images highlighted both the inner structure of cervix and the arrangement of cervical collagen fibers confirmed by the second harmonic generation microscopy. The statistical analysis and two-Gaussians fit of the distributions of linear retardance and linear depolarization in the entire images of cervical tissue (without manual selection of the specific regions of interest) quantified the randomization of collagen fibers alignment with gestation time. At day 18 the remodeling of cervical extracellular matrix of collagen was measurable at the external cervical os that is available for the direct optical observations in vivo. It supports the assumption that imaging Mueller polarimetry holds promise for the fast and accurate collagen scoring in pregnancy and the assessment of the preterm birth risk.
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妊娠期间宫颈基质的机械性能与细胞外基质成分之间的关系。
影响因子：
3.4
作者：
House M;Kaplan DL;Socrate S
通讯作者：
Socrate S
影响因子：
2.3
作者：
Feltovich, Helen;Nam, Kibo;Hall, Timothy J.
通讯作者：
Hall, Timothy J.
影响因子：
3.5
作者：
Ghosh, Nirmalya;Vitkin, I. Alex
通讯作者：
Vitkin, I. Alex
DOI：
10.1364/josaa.13.001106
发表时间：
1996-05-01
影响因子：
1.9
作者：
Lu, SY;Chipman, RA
通讯作者：
Chipman, RA
影响因子：
7.2
作者：
Iams, JD
通讯作者：
Iams, JD