An Interspecies Comparative Analysis of the Predicted Secretomes of the Necrotrophic Plant Pathogens Sclerotinia sclerotiorum and Botrytis cinerea.
An Interspecies Comparative Analysis of the Predicted Secretomes of the Necrotrophic Plant Pathogens Sclerotinia sclerotiorum and Botrytis cinerea.
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对坏死性植物病原体的菌核病菌核和辣椒粉预测的分析的种间比较分析。
DOI：
10.1371/journal.pone.0130534
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Hammond-Kosack K
中科院分区：
文献类型：
--
作者：
Heard S;Brown NA;Hammond-Kosack K
植物病原真菌与寄主植物形成密切联系并引起疾病。为了成功，真菌病原体通过分泌蛋白质效应物、水解酶和代谢物与易感宿主进行交流。菌核菌（Sclerotinia sclerotiorum）和灰霉病菌（Botrytis cinerea）是经济上重要的坏死性真菌病原体，可引起许多作物物种的疾病。本研究利用强大的生物信息学管道对菌核葡萄球菌和灰葡萄球菌的精制分泌组进行预测，分别鉴定出432个和499个蛋白。对菌丝病的分析表明，16%的分泌组编码基因位于小的、序列异质的基因簇中，分布在16条预测染色体中的13条上。功能分析强调了植物细胞水解、氧化还原过程和氧化还原状态对菌核芽孢杆菌和灰绿芽孢杆菌分泌体和潜在宿主感染的重要性。只有8%的预测蛋白在两个分泌组中是不同的。与菌核葡萄球菌相比，葡萄球菌分泌组缺乏含有CFEM或lysm的蛋白质。通过对115种真菌和卵菌基因组的比较，鉴定出30种菌核菌和灰绿杆菌特有的蛋白质，外加11种菌核菌特有的蛋白质和32种灰绿杆菌特有的蛋白质。表达序列标签（EST）和蛋白质组学分析表明，246个菌核丝虫病分泌组编码基因具有EST支持，其中101个基因仅在体外表达，49个基因仅在植物中表达，42个预测蛋白在实验中被证明是分泌的。这些对两种重要的坏死性病原体的详细的计算机分析将允许在选择候选效应蛋白进行植物功能分析时做出明智的选择。
Phytopathogenic fungi form intimate associations with host plant species and cause disease. To be successful, fungal pathogens communicate with a susceptible host through the secretion of proteinaceous effectors, hydrolytic enzymes and metabolites. Sclerotinia sclerotiorum and Botrytis cinerea are economically important necrotrophic fungal pathogens that cause disease on numerous crop species. Here, a powerful bioinformatics pipeline was used to predict the refined S. sclerotiorum and B. cinerea secretomes, identifying 432 and 499 proteins respectively. Analyses focusing on S. sclerotiorum revealed that 16% of the secretome encoding genes resided in small, sequence heterogeneous, gene clusters that were distributed over 13 of the 16 predicted chromosomes. Functional analyses highlighted the importance of plant cell hydrolysis, oxidation-reduction processes and the redox state to the S. sclerotiorum and B. cinerea secretomes and potentially host infection. Only 8% of the predicted proteins were distinct between the two secretomes. In contrast to S. sclerotiorum, the B. cinerea secretome lacked CFEM- or LysM-containing proteins. The 115 fungal and oomycete genome comparison identified 30 proteins specific to S. sclerotiorum and B. cinerea, plus 11 proteins specific to S. sclerotiorum and 32 proteins specific to B. cinerea. Expressed sequence tag (EST) and proteomic analyses showed that 246 S. sclerotiorum secretome encoding genes had EST support, including 101 which were only expressed in vitro and 49 which were only expressed in planta, whilst 42 predicted proteins were experimentally proven to be secreted. These detailed in silico analyses of two important necrotrophic pathogens will permit informed choices to be made when candidate effector proteins are selected for function analyses in planta.
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通讯作者：
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