Metagenomics Reveals Microbial Diversity and Metabolic Potentials of Seawater and Surface Sediment From a Hadal Biosphere at the Yap Trench.
Metagenomics Reveals Microbial Diversity and Metabolic Potentials of Seawater and Surface Sediment From a Hadal Biosphere at the Yap Trench.
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宏基因组学揭示了雅浦海沟深渊生物圈海水和表层沉积物的微生物多样性和代谢潜力
DOI：
10.3389/fmicb.2018.02402
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发表时间：
2018
影响因子：
5.2
通讯作者：
Li M
中科院分区：
文献类型：
--
作者：
Zhang X;Xu W;Liu Y;Cai M;Luo Z;Li M
哈达尔生物圈代表海洋最深的部分，水深6,000米。越来越多的证据表明，在这个环境中存在着以异养过程为主的独特微生物群落。然而，由于样品采集的技术限制，对微生物多样性及其代谢潜力的研究受到限制。在此，我们对西太平洋YAP海沟5,000-6,000米水深的三个海水样本和4,435-6,578米水深的三个表层沉积物样本进行了详细的微基因组分析。不同的微生物群落组成不同，在海水中以伽马蛋白细菌为主，在表层沉积物中以塔姆考古菌为主。对碳、氮和硫代谢相关基因的比较分析表明，异养过程(即碳水化合物、碳氢化合物和芳香烃的降解)是海水中最常见的微生物代谢过程，而化学自养代谢过程，如氨氧化和HP/HB循环固定二氧化碳，可能主导了YAP海沟的表层沉积物群落。此外，在海水和沉积物中都检测到了丰富的与胁迫反应和金属抗性相关的基因，因此进一步推测，金属抗性基因的丰富是HADAL微生物群落的特征。总体而言，这项研究揭示了YAP海沟微生物的代谢多样性，它们在碳、氮和硫生物地球化学循环中的作用，以及它们如何适应这种独特的哈达尔环境。
Hadal biosphere represents the deepest part of the ocean with water depth >6,000 m. Accumulating evidence suggests the existence of unique microbial communities dominated by heterotrophic processes in this environment. However, investigations of the microbial diversity and their metabolic potentials are limited because of technical constraints for sample collection. Here, we provide a detailed metagenomic analysis of three seawater samples at water depths 5,000–6,000 m below sea level (mbsl) and three surface sediment samples at water depths 4,435–6,578 mbsl at the Yap Trench of the western Pacific. Distinct microbial community compositions were observed with the dominance of Gammaproteobacteria in seawater and Thaumarchaeota in surface sediment. Comparative analysis of the genes involved in carbon, nitrogen and sulfur metabolisms revealed that heterotrophic processes (i.e., degradation of carbohydrates, hydrocarbons, and aromatics) are the most common microbial metabolisms in the seawater, while chemolithoautotrophic metabolisms such as ammonia oxidation with the HP/HB cycle for CO2 fixation probably dominated the surface sediment communities of the Yap Trench. Furthermore, abundant genes involved in stress response and metal resistance were both detected in the seawater and sediments, thus the enrichment of metal resistance genes is further hypothesized to be characteristic of the hadal microbial communities. Overall, this study sheds light on the metabolic versatility of microorganisms in the Yap Trench, their roles in carbon, nitrogen, and sulfur biogeochemical cycles, and how they have adapted to this unique hadal environment.
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影响因子：
10.7
作者：
Minh BQ;Nguyen MA;von Haeseler A
通讯作者：
von Haeseler A
影响因子：
14.9
作者：
Benson DA;Cavanaugh M;Clark K;Karsch-Mizrachi I;Lipman DJ;Ostell J;Sayers EW
通讯作者：
Sayers EW
影响因子：
2.7
作者：
Kang DD;Froula J;Egan R;Wang Z
通讯作者：
Wang Z
对大量人类肠道微生物群中抗生素抗性基因进行宏基因组分析
影响因子：
16.6
作者：
Hu, Yongfei;Yang, Xi;Zhu, Baoli
通讯作者：
Zhu, Baoli
DOI：
10.1073/pnas.0907176107
发表时间：
2010-05-11
影响因子：
11.1
作者：
Bar-Even, Arren;Noor, Elad;Milo, Ron
通讯作者：
Milo, Ron