Genome-wide association study dissects the genetic architecture of seed weight and seed quality in rapeseed (Brassica napus L.).
Genome-wide association study dissects the genetic architecture of seed weight and seed quality in rapeseed (Brassica napus L.).
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DOI：
10.1093/dnares/dsu002
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发表时间：
2014-08
期刊：
影响因子：
--
通讯作者：
Wu X
中科院分区：
文献类型：
--
作者：
Li F;Chen B;Xu K;Wu J;Song W;Bancroft I;Harper AL;Trick M;Liu S;Gao G;Wang N;Yan G;Qiao J;Li J;Li H;Xiao X;Zhang T;Wu X
关联作图可以快速、高效地剖析复杂的农艺性状。油菜是经济上最重要的多倍体油料作物之一，但其基因组序列尚未公布。在这项研究中，利用最近开发的60K Brassica Infinium®SNP阵列对472份材料的关联小组进行了分析。利用代表油菜基因组的‘伪分子’对该阵列的单核苷酸多态(SNPs)进行电子作图，以建立它们的假设顺序，并进行种子重量和种子质量的关联作图。结果表明，芥酸含量与甘蓝型油菜A8和C3上的芥酸含量存在显著关联，SNPs峰值距离关键基因Bna A.FAE1和Bna C.FAE1分别仅233和128 kb。BnaA.FAE1也被发现与含油量显著相关。在A9、C2、C7和C9上与硫代葡萄糖苷含量相关的四个SNPs簇附近发现了拟南芥HAG1的同源基因。在粒重方面，我们在A7和A9上检测到两个关联信号，这与以前的数量性状座位定位研究一致。结果表明，该关联作图方法适用于油菜复杂性状的精细定位。
Association mapping can quickly and efficiently dissect complex agronomic traits. Rapeseed is one of the most economically important polyploid oil crops, although its genome sequence is not yet published. In this study, a recently developed 60K Brassica Infinium® SNP array was used to analyse an association panel with 472 accessions. The single-nucleotide polymorphisms (SNPs) of the array were in silico mapped using ‘pseudomolecules’ representative of the genome of rapeseed to establish their hypothetical order and to perform association mapping of seed weight and seed quality. As a result, two significant associations on A8 and C3 of Brassica napus were detected for erucic acid content, and the peak SNPs were found to be only 233 and 128 kb away from the key genes BnaA.FAE1 and BnaC.FAE1. BnaA.FAE1 was also identified to be significantly associated with the oil content. Orthologues of Arabidopsis thaliana HAG1 were identified close to four clusters of SNPs associated with glucosinolate content on A9, C2, C7 and C9. For seed weight, we detected two association signals on A7 and A9, which were consistent with previous studies of quantitative trait loci mapping. The results indicate that our association mapping approach is suitable for fine mapping of the complex traits in rapeseed.
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影响因子：
4.4
作者：
Delourme R;Falentin C;Fomeju BF;Boillot M;Lassalle G;André I;Duarte J;Gauthier V;Lucante N;Marty A;Pauchon M;Pichon JP;Ribière N;Trotoux G;Blanchard P;Rivière N;Martinant JP;Pauquet J
通讯作者：
Pauquet J
影响因子：
13.8
作者：
Augustine, Rehna;Mukhopadhyay, Arundhati;Bisht, Naveen C.
通讯作者：
Bisht, Naveen C.
影响因子：
3.1
作者：
Howell, PM;Sharpe, AG;Lydiate, DJ
通讯作者：
Lydiate, DJ
影响因子：
5.8
作者：
Bradbury, Peter J.;Zhang, Zhiwu;Buckler, Edward S.
通讯作者：
Buckler, Edward S.
影响因子：
5.4
作者：
Bus, Anja;Koerber, Niklas;Stich, Benjamin
通讯作者：
Stich, Benjamin