Genome-wide expression and network analyses of mutants in key brassinosteroid signaling genes.
Genome-wide expression and network analyses of mutants in key brassinosteroid signaling genes.
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DOI：
10.1186/s12864-021-07778-w
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发表时间：
2021-06-22
期刊：
影响因子：
4.4
通讯作者：
Li J
中科院分区：
文献类型：
--
作者：
Seyed Rahmani R;Shi T;Zhang D;Gou X;Yi J;Miclotte G;Marchal K;Li J
类维生素B1（BR）信号与其他信号通路共同调控植物的生长发育。虽然许多基因已被确定在BR信号中发挥作用，但破坏这些关键BR基因的生物学和功能后果仍需要详细的调查。在这里，我们进行了表型和转录组比较A。在BRI 1基因中携带功能丧失突变的拟南芥品系bri 1 -5，其表现出矮化表型，以及其三个激活标签抑制子品系，其能够部分地将bri 1 -5突变表型恢复为WS 2表型，即bri 1 -5/bri 1 -1D、bri 1 -5/brs 1 -1D和bri 1 -5/bak 1 -1D。在所研究的三个bri 1 -5抑制子中，bri 1 -5/bak 1 -1D是在转录水平上最有效的抑制子。所有三个bri 1 -5抑制基因的表达在脱落酸（阿坝信号）途径的改变，表明阿坝可能有助于野生型表型的部分恢复，在这些bri 1 -5抑制。网络分析显示BR和其他植物激素信号通路之间的串扰，表明干扰一种激素信号通路会影响其他激素信号通路。此外，差异表达分析表明BR信号对BR生物合成存在强烈的负反馈，并预测BRS 1可能负责为BRI 1与其配体之间的相互作用提供最佳环境，而不是直接参与信号传导。我们的研究提供了关键油菜素类固醇（BR）信号基因，特别是BRS 1的分子机制和功能的见解。在线版本包含补充材料，可通过10.1186/s12864-021-07778-w获得。
Brassinosteroid (BR) signaling regulates plant growth and development in concert with other signaling pathways. Although many genes have been identified that play a role in BR signaling, the biological and functional consequences of disrupting those key BR genes still require detailed investigation. Here we performed phenotypic and transcriptomic comparisons of A. thaliana lines carrying a loss-of-function mutation in BRI1 gene, bri1–5, that exhibits a dwarf phenotype and its three activation-tag suppressor lines that were able to partially revert the bri1–5 mutant phenotype to a WS2 phenotype, namely bri1–5/bri1–1D, bri1–5/brs1–1D, and bri1–5/bak1–1D. From the three investigated bri1–5 suppressors, bri1–5/bak1–1D was the most effective suppressor at the transcriptional level. All three bri1–5 suppressors showed altered expression of the genes in the abscisic acid (ABA signaling) pathway, indicating that ABA likely contributes to the partial recovery of the wild-type phenotype in these bri1–5 suppressors. Network analysis revealed crosstalk between BR and other phytohormone signaling pathways, suggesting that interference with one hormone signaling pathway affects other hormone signaling pathways. In addition, differential expression analysis suggested the existence of a strong negative feedback from BR signaling on BR biosynthesis and also predicted that BRS1, rather than being directly involved in signaling, might be responsible for providing an optimal environment for the interaction between BRI1 and its ligand. Our study provides insights into the molecular mechanisms and functions of key brassinosteroid (BR) signaling genes, especially BRS1. The online version contains supplementary material available at 10.1186/s12864-021-07778-w.
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影响因子：
64.8
作者：
Chinchilla, Delphine;Zipfel, Cyril;Boller, Thomas
通讯作者：
Boller, Thomas
影响因子：
7.4
作者：
Goda, H;Shimada, Y;Yoshida, S
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Yoshida, S
影响因子：
6.4
作者：
Kim, Sun Young;Kim, Beg Hab;Nam, Kyoung Hee
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Nam, Kyoung Hee
影响因子：
7.4
作者：
Goda, H;Sawa, S;Yoshida, S
通讯作者：
Yoshida, S
影响因子：
5.6
作者：
Gruszka D
通讯作者：
Gruszka D