Induced temperature-dependent DNA degradation by C60 without photoactivation
Induced temperature-dependent DNA degradation by C60 without photoactivation
复制标题
C60 诱导温度依赖性 DNA 降解，无需光活化
DOI：
10.1007/s11434-011-4694-6
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发表时间：
2011
影响因子：
--
通讯作者：
G. Jiang
中科院分区：
文献类型：
--
作者：
Chang Wang;Yan Lin;Ying Wang;Yong Liang;L. Meng;Jie Zhang;Qunfang Zhou;G. Jiang
本研究表明，C60可在室温下降解pBR322质粒DNA，无需光激活。提高培养温度、反应时间和C60浓度均能促进降解。我们还发现在C60溶液中形成了超氧自由基（O2−·）。超氧化物歧化酶显著抑制C60诱导的DNA裂解和O2−·生成。这些结果表明，在室温下，C60诱导的活性氧的形成引起了DNA的分裂。此外，我们证明了DNA降解被酸酰胺化学物质（如甲酰胺）显著抑制，并通过增加反应溶液的离子强度。这些结果表明，DNA的构象稳定性和C60的表面性质是调控DNA降解的重要因素。我们认为C60在室温下可以结合DNA，降低DNA构象的稳定性，诱导活性氧的形成和DNA的裂解。我们的研究结果为C60的遗传毒性提供了一种可能的解释，这应该在未来使用这种特殊的纳米材料时加以考虑。
This study shows that C60 can degrade pBR322 plasmid DNA at room temperature without photoactivation. The degradation was enhanced by increasing incubation temperature, reaction time or C60 concentration. We also found that superoxide radical anions (O2−·) were formed in the C60 solution. Superoxide dismutase significantly inhibited DNA cleavage and O2−· generation induced by C60. These results suggest that DNA cleavage was caused by the formation of reactive oxygen species induced by C60 at room temperature. Furthermore, we demonstrate that the DNA degradation was significantly inhibited by acid amide chemicals such as formamide, and by increased ionic strength of the reaction solution. These results indicate that the DNA conformation stability and the surface properties of C60 are important factors regulating DNA degradation. We propose that C60 can bind DNA, decrease DNA conformation stability, and induce the formation of reactive oxygen species and DNA cleavage at room temperature. Our results provide a possible explanation for the genotoxicity of C60, which should be considered in future use of this particular nano-material.
影响因子：
14.9
作者：
Chakrabarti, R;Schutt, CE
通讯作者：
Schutt, CE
影响因子：
3.8
作者：
Usenko, Crystal Y.;Harper, Stacey L.;Tanguay, Robert L.
通讯作者：
Tanguay, Robert L.
影响因子：
3.5
作者：
Chakrabarti, R;Schutt, CE
通讯作者：
Schutt, CE