An Incoherent Magnetic Field Inhibited EGF Receptor Clustering and Phosphorylation Induced by a 50-Hz Magnetic Field in Cultured FL Cells
An Incoherent Magnetic Field Inhibited EGF Receptor Clustering and Phosphorylation Induced by a 50-Hz Magnetic Field in Cultured FL Cells
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不相干磁场抑制培养 FL 细胞中 50 Hz 磁场诱导的 EGF 受体聚集和磷酸化
DOI：
10.1159/000185524
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发表时间：
2008-12
影响因子：
--
通讯作者：
中科院分区：
文献类型：
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作者：
以前，我们发现，暴露于50 Hz的磁场（MF）在0.4 mT可以诱导表皮生长因子（EGF）受体聚集在中国仓鼠肺（CHL）成纤维细胞和叠加的非相干MF具有相同的强度可以抑制效果。在本实验中，我们研究了50-Hz MF暴露在不同强度的EGF受体聚集和人羊膜细胞（FL）的磷酸化的影响，并探讨了不连贯的MF的相互作用。分别用免疫荧光共聚焦显微镜和免疫印迹技术分析细胞膜表面EGF受体的聚集和磷酸化。EGF治疗作为阳性对照。结果表明，与假暴露相比，0.1，0.2或0.4mT的50 Hz磁场暴露15 min可显著诱导FL细胞EGF受体聚集，并增强酪氨酸1173残基的磷酸化，而0.05mT磁场暴露15 min则无明显影响。暴露于0.2 mT的非相干MF（频率范围在30至90 Hz之间），同时既不诱导EGF受体聚集，也不增强FL细胞中EGF受体的磷酸化。当叠加时，在0.2 mT的非相干MF完全抑制EGF受体聚集和磷酸化诱导的50-Hz MF在0.1和0.2 mT。然而，非相干MF不能完全消除由0.4 mT 50 Hz MF引起的影响。基于本实验的结果，我们得出结论，膜受体可能是极低频（ELF）MF与细胞相互作用的主要靶点之一，并且在EGF受体的情况下，强度阈值在0.05和0.1 mT之间。非相干MF可以完全抑制等强度或更低强度的ELF-MF引起的效应。
Previously, we found that exposure to a 50-Hz magnetic field (MF) at 0.4 mT could induce epidermal growth factor (EGF) receptor clustering in Chinese hamster lung (CHL) fibroblast cells and superposition of an incoherent MF with the same intensity could inhibit the effect. In the present experiment, we investigated the effects of 50-Hz MF exposure at different intensities on EGF receptor clustering and phosphorylation in human amniotic cells (FL), and explored the interaction effect of an incoherent MF. Clustering and phosphorylation of EGF receptors on cellular membrane surface were analyzed using immunofluorescence assessed by confocal microscopy and western blot technology, respectively. EGF treatment served as a positive control. The results showed that, compared with sham exposure, exposure to a 50-Hz MF at 0.1, 0.2 or 0.4 mT for 15 min could significantly induce EGF receptor clustering and enhance phosphorylation on tyrosine-1173 residue in FL cells, whereas exposure to a 0.05 mT field for 15 min did not caused a significant effect. Exposure to an incoherent MF (frequency range between 30 to 90 Hz) at 0.2 mT for the same time neither induced EGF receptor clustering nor enhanced phosphorylation of EGF receptor in FL cells. When superposed, the incoherent MF at 0.2 mT completely inhibited EGF receptor clustering and phosphorylation induced by a 50-Hz MF at 0.1 and 0.2 mT. However, the incoherent MF could not completely eliminate the effects induced by a 0.4 mT 50-Hz MF. Based on the results of this experiment, we conclude that membrane receptors could be one of the main targets where extremely-low frequency (ELF) MF interacts with cells, and the intensity threshold, in the case of EGF receptors, is between 0.05 and 0.1 mT. An incoherent MF could completely inhibit the effects induced by an ELF-MF of equal or lower intensity.
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DOI：
--
发表时间：
2002-08
影响因子：
--
作者：
Wenjun Sun;H. Chiang;Yi-ti Fu;D. Lu;Zheng-ping Xu
通讯作者：
Wenjun Sun;H. Chiang;Yi-ti Fu;D. Lu;Zheng-ping Xu
DOI：
--
发表时间：
2000
期刊：
Biomedical sciences instrumentation
影响因子：
--
作者：
G. Nindl;W. Balcavage;D. Vesper;J. Swez;B. Wetzel;J. Chamberlain;M. T. Fox
通讯作者：
G. Nindl;W. Balcavage;D. Vesper;J. Swez;B. Wetzel;J. Chamberlain;M. T. Fox
DOI：
--
发表时间：
2002
期刊：
IARC monographs on the evaluation of carcinogenic risks to humans
影响因子：
--
作者：
Iarc Monographs
通讯作者：
Iarc Monographs
影响因子：
5
作者：
P. Demers;D. Thomas;D. Thomas;K. Rosenblatt;K. Rosenblatt;L. Jimenez;L. Jimenez;A. McTiernan;H. Stalsberg;A. Stemhagen;W. Thompson;M. G. Curnen;W. Satariano;D. Austin;P. Isacson;R. Greenberg;C. Key;L. Kolonel;D. West
通讯作者：
P. Demers;D. Thomas;D. Thomas;K. Rosenblatt;K. Rosenblatt;L. Jimenez;L. Jimenez;A. McTiernan;H. Stalsberg;A. Stemhagen;W. Thompson;M. G. Curnen;W. Satariano;D. Austin;P. Isacson;R. Greenberg;C. Key;L. Kolonel;D. West
DOI：
10.1016/0302-4598(96)05060-x
发表时间：
1996-08
期刊：
Bioelectrochemistry and Bioenergetics
影响因子：
--
作者：
P. Raskmark;S. Kwee
通讯作者：
P. Raskmark;S. Kwee