Widespread evidence for horizontal transfer of transposable elements across Drosophila genomes.
Widespread evidence for horizontal transfer of transposable elements across Drosophila genomes.
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DOI：
10.1186/gb-2009-10-2-r22
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发表时间：
2009-02-18
期刊：
影响因子：
12.3
通讯作者：
Maside X
中科院分区：
文献类型：
--
作者：
Bartolomé C;Bello X;Maside X
转座因子的全基因组比较揭示了三种果蝇之间出乎意料的高水平转移率的证据。水平转移（HT）可能在转座因子（TE）的长期存在中发挥重要作用，因为它为它们提供了避免宿主沉默机制和自然选择的检查效应的可能性，这最终会促使它们从基因组中消除。然而，尽管越来越多的证据HT的TE，其发生率之间的TE池的模式真核生物仍然是未知的。我们提取并比较了果蝇、黑腹果蝇和黑腹果蝇基因组中所有可能具有功能的自主TE的核苷酸序列。simulans和D. yakuba -1，436插入分为141个不同的家庭-和显示，在两个或两个以上的物种中发现的家庭的大部分显示水平的遗传分化和物种内的多样性，显着低于预期的假设拷贝数平衡和垂直传输，并符合最近的起源HT。长末端重复序列（LTR）反转录转座子形成近90%的HT病例。HT足迹在DNA转座子中也很常见（占比较家族的40%），但在非LTR逆转录元件中很少见（6%）。我们的研究结果表明，基因组率为0.04 HT事件每个家庭每百万年之间的三个物种的研究，以及元素的主要类别之间的显着变化。研究了D. melanogaster、黑腹杜父鱼D. simulans和D. yakuba表明，三分之一的TE家庭起源于这些物种之间的最近HT。这一结果强调了水平传播在果蝇TEs自然史中的重要作用。
A genome-wide comparison of transposable elements reveals evidence for unexpectedly high rates of horizontal transfer between three species of Drosophila Horizontal transfer (HT) could play an important role in the long-term persistence of transposable elements (TEs) because it provides them with the possibility to avoid the checking effects of host-silencing mechanisms and natural selection, which would eventually drive their elimination from the genome. However, despite the increasing evidence for HT of TEs, its rate of occurrence among the TE pools of model eukaryotic organisms is still unknown. We have extracted and compared the nucleotide sequences of all potentially functional autonomous TEs present in the genomes of Drosophila melanogaster, D. simulans and D. yakuba - 1,436 insertions classified into 141 distinct families - and show that a large fraction of the families found in two or more species display levels of genetic divergence and within-species diversity that are significantly lower than expected by assuming copy-number equilibrium and vertical transmission, and consistent with a recent origin by HT. Long terminal repeat (LTR) retrotransposons form nearly 90% of the HT cases detected. HT footprints are also frequent among DNA transposons (40% of families compared) but rare among non-LTR retroelements (6%). Our results suggest a genomic rate of 0.04 HT events per family per million years between the three species studied, as well as significant variation between major classes of elements. The genome-wide patterns of sequence diversity of the active autonomous TEs in the genomes of D. melanogaster, D. simulans and D. yakuba suggest that one-third of the TE families originated by recent HT between these species. This result emphasizes the important role of horizontal transmission in the natural history of Drosophila TEs.
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影响因子：
64.8
作者：
Clark, Andrew G.;Eisen, Michael B.;MacCallum, Iain
通讯作者：
MacCallum, Iain
影响因子：
3.3
作者：
Biedler, James K.;Sha, Hongguang;Tu, Zhijian
通讯作者：
Tu, Zhijian
种群基因组学：果蝇果蝇中多态性和差异的全基因组分析。
影响因子：
9.8
作者：
Begun, David J.;Holloway, Alisha K.;Stevens, Kristian;Hillier, LaDeana W.;Poh, Yu-Ping;Hahn, Matthew W.;Nista, Phillip M.;Jones, Corbin D.;Kern, Andrew D.;Dewey, Colin N.;Pachter, Lior;Myers, Eugene;Langley, Charles H.
通讯作者：
Langley, Charles H.
DOI：
10.1073/pnas.0702552104
发表时间：
2007-07-03
影响因子：
11.1
作者：
Bergman, Casey M.;Bensasson, Douda
通讯作者：
Bensasson, Douda
影响因子：
1.5
作者：
Bartolomé, C;Maside, X
通讯作者：
Maside, X