Comparative analysis of root transcriptome profiles of two pairs of drought-tolerant and susceptible rice near-isogenic lines under different drought stress.
Comparative analysis of root transcriptome profiles of two pairs of drought-tolerant and susceptible rice near-isogenic lines under different drought stress.
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DOI：
10.1186/1471-2229-11-174
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发表时间：
2011-12-02
影响因子：
5.3
通讯作者：
Kikuchi S
中科院分区：
文献类型：
--
作者：
Moumeni A;Satoh K;Kondoh H;Asano T;Hosaka A;Venuprasad R;Serraj R;Kumar A;Leung H;Kikuchi S
植物根系是吸收土壤水分和养分的重要器官，是感知和传递土壤水分亏缺信号到地上部的重要器官。目前对水稻干旱胁迫转录本的研究大多依赖于干旱条件下不同遗传背景的比较研究。一种更可靠的方法是使用近等基因系(NILs)，它们具有共同的遗传背景，但在初始缺水情况下对干旱胁迫的抗性水平不同。在这里，我们研究了IR64背景中的两对近等基因系，它们的耐旱性存在差异。获得了不同程度干旱胁迫下水稻近等基因系根系的基因表达谱，有助于识别干旱胁迫相关基因及其机制。全球基因表达分析表明，大约55%的基因在水稻根中差异表达，以响应干旱胁迫处理。近等基因系差异表达基因(Deg)的数量随着水分亏缺程度的增加而增加，从轻度干旱胁迫到重度干旱胁迫，表明更多的基因受到干旱胁迫的影响。基因本体(GO)分析和生物通径分析表明，近等基因系IR77298-14-1-2-B-10和IR77298-5-6-B-18中的激活基因主要参与次生代谢、氨基酸代谢、对刺激的反应、防御反应、转录和信号转导，下调的基因主要参与光合作用和细胞壁的生长。我们还观察到赤霉酸(GA)和生长素串扰对耐性近等基因系侧根形成的调控作用。对两对遗传背景相同(~97%)的近等基因系的转录组分析表明，它们的基因表达谱存在明显差异，可以有效地解开与抗旱相关的基因。与耐性中等的近等基因系IR77298-5-6-B-18和其他敏感近等基因系相比，耐性近等基因系IR77298-14-1-2-B-10在细胞生长、激素生物合成、细胞运输、氨基酸代谢、信号转导、转录因子和碳水化合物代谢等方面对干旱胁迫的响应表现出更多的DEG。因此，在这两个耐受系中，不同的机制正在实现宽容。
Plant roots are important organs to uptake soil water and nutrients, perceiving and transducing of soil water deficit signals to shoot. The current knowledge of drought stress transcriptomes in rice are mostly relying on comparative studies of diverse genetic background under drought. A more reliable approach is to use near-isogenic lines (NILs) with a common genetic background but contrasting levels of resistance to drought stress under initial exposure to water deficit. Here, we examined two pairs of NILs in IR64 background with contrasting drought tolerance. We obtained gene expression profile in roots of rice NILs under different levels of drought stress help to identify genes and mechanisms involved in drought stress. Global gene expression analysis showed that about 55% of genes differentially expressed in roots of rice in response to drought stress treatments. The number of differentially expressed genes (DEGs) increased in NILs as the level of water deficits, increased from mild to severe condition, suggesting that more genes were affected by increasing drought stress. Gene onthology (GO) test and biological pathway analysis indicated that activated genes in the drought tolerant NILs IR77298-14-1-2-B-10 and IR77298-5-6-B-18 were mostly involved in secondary metabolism, amino acid metabolism, response to stimulus, defence response, transcription and signal transduction, and down-regulated genes were involved in photosynthesis and cell wall growth. We also observed gibberellic acid (GA) and auxin crosstalk modulating lateral root formation in the tolerant NILs. Transcriptome analysis on two pairs of NILs with a common genetic background (~97%) showed distinctive differences in gene expression profiles and could be effective to unravel genes involved in drought tolerance. In comparison with the moderately tolerant NIL IR77298-5-6-B-18 and other susceptible NILs, the tolerant NIL IR77298-14-1-2-B-10 showed a greater number of DEGs for cell growth, hormone biosynthesis, cellular transports, amino acid metabolism, signalling, transcription factors and carbohydrate metabolism in response to drought stress treatments. Thus, different mechanisms are achieving tolerance in the two tolerant lines.
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