The ham-5, rcm-1 and rco-1 genes regulate hyphal fusion in Neurospora crassa.
The ham-5, rcm-1 and rco-1 genes regulate hyphal fusion in Neurospora crassa.
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DOI：
10.1099/mic.0.040147-0
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发表时间：
2010-09
期刊：
影响因子：
--
通讯作者：
Free SJ
中科院分区：
文献类型：
--
作者：
Aldabbous MS;Roca MG;Stout A;Huang IC;Read ND;Free SJ
突变体的粗糙脉孢菌不能参与营养菌丝融合（吻合）的分离和特点。从这个分析中，三个基因，rcm-1，rco-1和ham-5，被确定和显示为菌丝融合所需的。rcm-1和rco-1基因是酿酒酵母SSN 6和TUP 1基因的同源物，它们在酵母中编码二聚体转录因子。我们证明，在N。rcm-1和rco-1基因是菌丝融合和正常菌丝形态所必需的，并且影响无性和有性发育。ham-5基因编码一个1686个氨基酸的蛋白质，具有两个推测的WD 40结构域，其可能参与蛋白质-蛋白质相互作用。Ham-5缺失突变体具有降低的菌丝延伸速率和改变的菌丝形态，并且不能产生菌落起始期间菌丝融合所需的分生孢子吻合管。
Mutants of Neurospora crassa unable to participate in vegetative hyphal fusion (anastomosis) were isolated and characterized. From this analysis, three genes, rcm-1, rco-1 and ham-5, were identified and shown to be required for hyphal fusion. The rcm-1 and rco-1 genes are homologues of the Saccharomyces cerevisiae SSN6 and TUP1 genes, which encode a dimeric transcription factor in yeast. We demonstrate that in N. crassa the rcm-1 and rco-1 genes are required for hyphal fusion and normal hyphal morphology, and influence both asexual and sexual development. The ham-5 gene encodes a 1686 amino acid protein with two putative WD40 domains, which might participate in protein–protein interactions. ham-5 deletion mutants had a reduced rate of hyphal extension and altered hyphal morphology, and were unable to produce the conidial anastomosis tubes that are required for hyphal fusion during colony initiation.
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影响因子：
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作者：
Bowman, SM;Piwowar, A;Free, SJ
通讯作者：
Free, SJ
影响因子：
--
作者：
Fleissner, Andre;Glass, N. Louise
通讯作者：
Glass, N. Louise
影响因子：
--
作者：
Pandey, A;Roca, MG;Glass, NL
通讯作者：
Glass, NL
影响因子：
--
作者：
Engh, Ines;Wuertz, Christian;Kueck, Ulrich
通讯作者：
Kueck, Ulrich
影响因子：
2.8
作者：
Bowman, SM;Piwowar, A;Free, SJ
通讯作者：
Free, SJ