Transcriptomic landscape of breast cancers through mRNA sequencing.
Transcriptomic landscape of breast cancers through mRNA sequencing.
复制标题
DOI：
10.1038/srep00264
复制
发表时间：
2012
影响因子：
4.6
通讯作者：
Kumar, Rakesh
中科院分区：
文献类型：
--
作者：
Eswaran, Jeyanthy;Cyanam, Dinesh;Mudvari, Prakriti;Reddy, Sirigiri Divijendra Natha;Pakala, Suresh B.;Nair, Sujit S.;Florea, Liliana;Fuqua, Suzanne A. W.;Godbole, Sucheta;Kumar, Rakesh
乳腺癌是一种异质性疾病，遗传图谱不明确，这对诊断和治疗构成了重大挑战。通过大规模平行mRNA测序，我们从17个属于TNBC、Non-TNBC和her2阳性乳腺癌的个体人体组织中获得了12亿个reads，并首次定义了它们的综合数字转录组。令人惊讶的是，我们发现了大量新的和未注释的转录本，揭示了全球乳腺癌转录组适应。比较转录组学分析阐明了三个乳腺癌组之间的差异表达转录物，确定了几个新的乳腺癌调节因子。我们的研究还发现了共同的转录调控元件，如高度丰富的初级转录本，包括骨连接蛋白、RACK1、钙连蛋白、钙网蛋白、FTL和B2M，以及三组之间初级转录本中富集的“基因组热点”。因此，我们的研究打开了以前未开发的利基，可以更好地了解疾病和开发潜在的干预策略。
Breast cancer is a heterogeneous disease with a poorly defined genetic landscape, which poses a major challenge in diagnosis and treatment. By massively parallel mRNA sequencing, we obtained 1.2 billion reads from 17 individual human tissues belonging to TNBC, Non-TNBC, and HER2-positive breast cancers and defined their comprehensive digital transcriptome for the first time. Surprisingly, we identified a high number of novel and unannotated transcripts, revealing the global breast cancer transcriptomic adaptations. Comparative transcriptomic analyses elucidated differentially expressed transcripts between the three breast cancer groups, identifying several new modulators of breast cancer. Our study also identified common transcriptional regulatory elements, such as highly abundant primary transcripts, including osteonectin, RACK1, calnexin, calreticulin, FTL, and B2M, and “genomic hotspots” enriched in primary transcripts between the three groups. Thus, our study opens previously unexplored niches that could enable a better understanding of the disease and the development of potential intervention strategies.
登录
查看更多内容
RACK1通过激活RhoA/Rho激酶通路促进乳腺癌迁移/转移
影响因子：
3.8
作者：
Cao, Xi-Xi;Xu, Jing-Da;Liu, Xiu-Ping
通讯作者：
Liu, Xiu-Ping
影响因子：
5.3
作者：
Geyer FC;Lopez-Garcia MA;Lambros MB;Reis-Filho JS
通讯作者：
Reis-Filho JS
影响因子：
11.5
作者：
Carey, Lisa A.;Dees, E. Claire;Perou, Charles M.
通讯作者：
Perou, Charles M.
影响因子：
4.4
作者：
Hu, Zhiyuan;Fan, Cheng;Perou, Charles M.
通讯作者：
Perou, Charles M.
影响因子：
8.4
作者：
GUSTERSON, BA
通讯作者：
GUSTERSON, BA