The Cellular and Mechanical Basis for Response Characteristics of Identified Primary Afferents in the Rat Vibrissal System.
The Cellular and Mechanical Basis for Response Characteristics of Identified Primary Afferents in the Rat Vibrissal System.
复制标题
DOI：
10.1016/j.cub.2019.12.068
复制
发表时间：
2020-03-09
期刊：
影响因子：
9.2
通讯作者：
Hartmann, Mitra J. Z.
中科院分区：
文献类型：
--
作者：
Furuta, Takahiro;Bush, Nicholas E.;Yang, Anne En-Tzu;Ebara, Satomi;Miyazaki, Naoyuki;Murata, Kazuyoshi;Hirai, Daichi;Shibata, Ken-ichi;Hartmann, Mitra J. Z.
与我们对听觉和视觉系统中感受器的反应特性的理解相比，我们对触觉感觉基础的机械感受器反应只有有限的理解。在这里，我们利用大鼠触须（须）阵列的定型形态，研究识别的初级触觉传入的编码和转导特性。我们进行了体内轴突内记录和标记实验，以量化四种不同类型的机械感受器在触须毛囊：环窦默克尔，披针形，棒状和网脊领默克尔的响应特性。在这些类型中，只有环窦默克尔末梢表现出缓慢的适应特性。在所有类型中，反应幅度和起始反应潜伏期之间存在弱的反比关系。所有传入神经都表现出强烈的“角度调谐”，即，其反应幅度和潜伏期取决于触须的偏转角度。虽然以前的研究表明，这种调谐应与毛囊中的机械感受器的角度位置对齐，这种对齐只观察到默克尔传入;其他传入类型的角度调谐显示没有明确的对齐与机械感受器的位置。生物力学模型表明，这种调谐差异可能是由机械感受器的差异敏感性的力量直接沿着胡须长度。电子显微镜调查的默克尔结束和披针形的结束在环窦的水平揭示了独特的解剖特征，可能会促进这些差异的敏感性。本研究系统地整合了生物力学原理与初级传入末梢的解剖和形态特征，以描述塑造触觉神经表征的物理和细胞处理。使用轴突内记录，Furuta等人量化了晶须系统中四种不同类型的机械感受器的响应特性。每种类型有不同的响应幅度，lavelet，和角度调谐。机械建模和电子显微镜有助于解释传感器和组织形态方面的反应。
Compared to our understanding of the response properties of receptors in the auditory and visual systems, we have only a limited understanding of the mechanoreceptor responses that underlie tactile sensation. Here, we exploit the stereotyped morphology of the rat vibrissal (whisker) array to investigate coding and transduction properties of identified primary tactile afferents. We performed in vivo intra-axonal recording and labeling experiments to quantify response characteristics of four different types of identified mechanoreceptors in the vibrissal follicle: ring-sinus Merkel; lanceolate; clublike; and rete-ridge collar Merkel. Of these types, only ring-sinus Merkel endings exhibited slowly adapting properties. A weak inverse relationship between response magnitude and onset response latency was found across all types. All afferents exhibited strong ‘‘angular tuning,’’ i.e., their response magnitude and latency depended on the whisker’s deflection angle. Although previous studies suggested that this tuning should be aligned with the angular location of the mechanoreceptor in the follicle, such alignment was observed only for Merkel afferents; angular tuning of the other afferent types showed no clear alignment with mechanoreceptor location. Biomechanical modeling suggested that this tuning difference might be explained by mechanoreceptors’ differential sensitivity to the force directed along the whisker length. Electron microscopic investigations of Merkel endings and lanceolate endings at the level of the ring sinus revealed unique anatomical features that may promote these differential sensitivities. The present study systematically integrates biomechanical principles with the anatomical and morphological characterization of primary afferent endings to describe the physical and cellular processing that shapes the neural representation of touch. Using intra-axonal recordings, Furuta et al. quantified response properties of four different types of identified mechanoreceptors in the whisker system. Each type had different response magnitudes, latencies, and angular tunings. Mechanical modeling and electron microscopy helped explain the responses in terms of sensor and tissue morphology.
登录
查看更多内容
影响因子：
2.5
作者：
Ebara, S;Kumamoto, K;Rice, FL
通讯作者：
Rice, FL
影响因子：
64.5
作者：
Li L;Rutlin M;Abraira VE;Cassidy C;Kus L;Gong S;Jankowski MP;Luo W;Heintz N;Koerber HR;Woodbury CJ;Ginty DD
通讯作者：
Ginty DD
默克尔细胞转导并编码触觉刺激以驱动Aβ型冲动。
影响因子：
64.5
作者：
Ikeda R;Cha M;Ling J;Jia Z;Coyle D;Gu JG
通讯作者：
Gu JG
影响因子：
3.7
作者：
Belli, Hayley M.;Bresee, Chris S.;Hartmann, Mitra J. Z.
通讯作者：
Hartmann, Mitra J. Z.
影响因子：
7.9
作者：
Huet, Lucie A.;Rudnicki, John W.;Hartmann, Mitra J. Z.
通讯作者：
Hartmann, Mitra J. Z.