Inner dynein arms but not outer dynein arms require the activity of kinesin homologue protein KHP1(FLA10) to reach the distal part of flagella in Chlamydomonas.
Inner dynein arms but not outer dynein arms require the activity of kinesin homologue protein KHP1(FLA10) to reach the distal part of flagella in Chlamydomonas.
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内部动力蛋白臂而不是外动臂需要驱动蛋白同源蛋白KHP1（FLA10）的活性，才能到达衣原体中的鞭毛远端。
DOI：
10.1083/jcb.133.2.371
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发表时间：
1996-04
影响因子：
7.8
通讯作者：
Henderson, S
中科院分区：
文献类型：
--
作者：
Piperno, G;Mead, K;Henderson, S
内动力蛋白臂，而不是外动力蛋白臂，需要KHP1（FLA10）的活性才能到达轴突远端，然后才能结合到外双线微管。我们用免疫荧光显微镜分析了四鞭毛虫双核细胞内动力蛋白臂轻链p28（IDA4）和外动力蛋白臂中间链IC69（ODA6）对内动力蛋白臂和外动力蛋白臂的拯救作用。在双核体中，两个具有不同遗传背景的菌株共享细胞质。因此，野生型轴突前体被运输到突变轴突中并在突变轴突中组装以补充缺陷。含有p28的ida4-野生型二核子内动力蛋白臂的拯救逐渐从轴突远端开始，并随着时间的推移向近端延伸。相比之下，oda2野生型二核子的外动力蛋白臂的拯救逐渐沿着轴突的整个长度发生。在21℃时，含有p28的ida4fla10-fla10二核子内动力蛋白臂的恢复与在ida4-野生型二核子中观察到的恢复相似，而在32℃（一个对KHP1（FLA10）不允许的温度）时则被抑制。相比之下，在21℃和32℃时，oda2fla10-fla10二核子的外动力蛋白臂的修复与oda2-野生型二核子的修复相似，在32℃时未被抑制。轴突轴内部亚结构的定位需要激酶同源蛋白1的活性。
Inner dynein arms, but not outer dynein arms, require the activity of KHP1(FLA10) to reach the distal part of axonemes before binding to outer doublet microtubules. We have analyzed the rescue of inner or outer dynein arms in quadriflagellate dikaryons by immunofluorescence microscopy of p28(IDA4), an inner dynein arm light chain, or IC69(ODA6), an outer dynein arm intermediate chain. In dikaryons two strains with different genetic backgrounds share the cytoplasm. As a consequence, wild-type axonemal precursors are transported to and assembled in mutant axonemes to complement the defects. The rescue of inner dynein arms containing p28 in ida4-wild-type dikaryons progressively occurred from the distal part of the axonemes and with time was extended towards the proximal part. In contrast, the rescue of outer dynein arms in oda2-wild-type dikaryons progressively occurred along the entire length of the axoneme. Rescue of inner dynein arms containing p28 in ida4fla10-fla10 dikaryons was similar to the rescue observed in ida4-wild-type dikaryons at 21 degrees C, whereas it was inhibited at 32 degrees C, a nonpermissive temperature for KHP1(FLA10). In contrast, rescue of outer dynein arms in oda2fla10-fla10 dikaryons was similar to the rescue observed in oda2-wild-type dikaryons at both 21 degrees and 32 degrees C and was not inhibited at 32 degrees C. Positioning of substructures in the internal part of the axonemal shaft requires the activity of kinesin homologue protein 1.
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DOI：
10.1083/jcb.125.5.1109
发表时间：
1994-06
期刊：
The Journal of cell biology
影响因子：
--
作者：
Piperno G;Mead K;LeDizet M;Moscatelli A
通讯作者：
Moscatelli A
影响因子：
3.3
作者：
LEDIZET, M;PIPERNO, G
通讯作者：
PIPERNO, G
DOI：
10.1073/pnas.74.8.3456
发表时间：
1977-01-01
影响因子：
11.1
作者：
LUCK, D;PIPERNO, G;HUANG, B
通讯作者：
HUANG, B
影响因子：
3.3
作者：
LEDIZET, M;PIPERNO, G
通讯作者：
PIPERNO, G
影响因子：
7.8
作者：
KOZMINSKI, KG;BEECH, PL;ROSENBAUM, JL
通讯作者：
ROSENBAUM, JL