The Chlamydomonas kinesin-like protein FLA10 is involved in motility associated with the flagellar membrane.

The Chlamydomonas kinesin-like protein FLA10 is involved in motility associated with the flagellar membrane.
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DOI:
10.1083/jcb.131.6.1517
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发表时间:
1995-12
影响因子:
7.8
通讯作者:
ROSENBAUM, JL
ROSENBAUM, JL
中科院分区:
生物学1区
文献类型:
--
作者:
KOZMINSKI, KG;BEECH, PL;ROSENBAUM, JL

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衣原体FLA 10基因显示编码鞭毛驱动蛋白样蛋白(Walther,Z.,M. Vashishtha和J.L.大厅1994. 126:175-188)。通过使用FLA 10的温度敏感性等位基因,我们已经确定FLA 10蛋白对于聚苯乙烯珠在鞭毛膜上的双向运动和鞭毛内运输(IFT)(鞭毛膜下的颗粒状颗粒的双向运动)都是必需的(Kozminski,K.G.,K.A.约翰逊、P.福舍尔和J.L.罗森鲍姆1993. Proc. Natl. Acad. Sci.(美国)。90:5519-5523)。此外,我们已经相关的IFT颗粒的存在和位置可视化的光学显微镜下观察到的电子致密复合物(筏)的鞭毛膜下的电子显微镜。FLA 10在膜下或鞭毛表面运动的作用也强烈支持的免疫定位的FLA 10之间的区域的轴丝外双微管和鞭毛膜。
The Chlamydomonas FLA10 gene was shown to encode a flagellar kinesin- like protein (Walther, Z., M. Vashishtha, and J.L. Hall. 1994. J. Cell Biol. 126:175-188). By using a temperature-sensitive allele of FLA10, we have determined that the FLA10 protein is necessary for both the bidirectional movement of polystyrene beads on the flagellar membrane and intraflagellar transport (IFT), the bidirectional movement of granule-like particles beneath the flagellar membrane (Kozminski, K.G., K.A. Johnson, P. Forscher, and J.L. Rosenbaum. 1993. Proc. Natl. Acad. Sci. (USA). 90:5519-5523). In addition, we have correlated the presence and position of the IFT particles visualized by light microscopy with that of the electron dense complexes (rafts) observed beneath the flagellar membrane by electron microscopy. A role for FLA10 in submembranous or flagellar surface motility is also strongly supported by the immunolocalization of FLA10 to the region between the axonemal outer doublet microtubules and the flagellar membrane.
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