Histologically Confirmed Recellularization is a Key Factor that Affects Meniscal Healing in Immature and Mature Meniscal Tears.
Histologically Confirmed Recellularization is a Key Factor that Affects Meniscal Healing in Immature and Mature Meniscal Tears.
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组织学证实的再细胞化是影响未成熟和成熟半月板撕裂半月板愈合的关键因素
DOI：
10.3389/fcell.2021.793820
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发表时间：
2021
影响因子：
5.5
通讯作者：
Ao Y
中科院分区：
文献类型：
--
作者：
Yan W;Dai W;Cheng J;Fan Y;Zhao F;Li Y;Maimaitimin M;Cao C;Shao Z;Li Q;Liu Z;Hu X;Ao Y
年轻人的尿道修复术的愈合结果优于老年人。然而，从组织学角度来看，年轻人上级愈合潜力的确切机制仍不清楚。本研究包括24只未成熟家兔和24只成熟家兔。在每只家兔的右膝内侧半月板前角制造撕裂。在术后1、3、6和12周处死动物。我们进行了宏观和组织学评价后修复标本。对感兴趣区域内的细胞进行计数，以确认未成熟泪腺中泪液部位的细胞化。测量细胞死亡带的宽度，以确定成熟睾丸中细胞死亡的区域。TUNEL法检测细胞凋亡。通过CD31免疫荧光评估血管化。通过计算相应组织学标本的平均光密度来评价糖胺聚糖和1型和2型胶原的含量。还评价了软骨变性。未成熟组在未治疗的尿道撕裂后的愈合结果是上级的。在未成熟的泪腺中，在泪腺边缘观察到具有类似于海绵状细胞形态的再细胞化。在关节囊附近观察到上级再细胞化，而远离关节囊的部位观察到的再细胞化较好。在成熟组中，撕裂部位没有发生再细胞化，但是我们观察到细胞死亡区逐渐扩大。未成熟和成熟泪腺在未处理的泪腺撕裂后1、3、6、12周出现细胞凋亡。我们研究了未成熟泪腺沿着撕裂边缘的血管化。糖胺聚糖和2型胶原沉积在未成熟的胰腺中受到负面影响。我们观察到糖胺聚糖降解在成熟的股骨髁和软骨退变，特别是在未成熟的股骨髁软骨。总之，与成熟兔相比，未成熟兔在未经治疗的结膜撕裂后表现出更强的愈合反应。血管化有助于在未成熟的膀胱破裂后的再细胞化。半月板损伤从根本上改变细胞外基质沉积。
Healing outcomes of meniscal repair are better in younger than in older. However, exact mechanisms underlying superior healing potential in younger remain unclear from a histological perspective. This study included 24 immature rabbits and 24 mature rabbits. Tears were created in the anterior horn of medial meniscus of right knee in each rabbit. Animals were sacrificed at 1, 3, 6, and 12 weeks postoperatively. We performed macroscopic and histological evaluations of post-meniscal repair specimens. Cells were counted within a region of interest to confirm cellularization at tear site in immature menisci. The width of cell death zone was measured to determine the region of cell death in mature menisci. Apoptosis was evaluated by TUNEL assay. Vascularization was assessed by CD31 immunofluorescence. The glycosaminoglycans and the types 1 and 2 collagen content was evaluated by calculating average optical density of corresponding histological specimens. Cartilage degeneration was also evaluated. Healing outcomes following untreated meniscal tears were superior in immature group. Recellularization with meniscus-like cell morphology was observed at tear edge in immature menisci. Superior recellularization was observed at meniscal sites close to joint capsule than at sites distant from the capsule. Recellularization did not occur at tear site in mature group; however, we observed gradual enlargement of cell death zone. Apoptosis was presented at 1, 3, 6, 12 weeks in immature and mature menisci after untreated meniscal tears. Vascularization was investigated along the tear edges in immature menisci. Glycosaminoglycans and type 2 collagen deposition were negatively affected in immature menisci. We observed glycosaminoglycan degradation in mature menisci and cartilage degeneration, specifically in immature cartilage of the femoral condyle. In conclusion, compared with mature rabbits, immature rabbits showed more robust healing response after untreated meniscal tears. Vascularization contributed to the recellularization after meniscal tears in immature menisci. Meniscal injury fundamentally alters extracellular matrix deposition.
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DOI：
10.1177/0363546516664889
发表时间：
2017-01
期刊：
The American journal of sports medicine
影响因子：
--
作者：
Brophy RH;Sandell LJ;Rai MF
通讯作者：
Rai MF
膝骨关节炎内侧半月板撕裂加重的三维有限元分析
影响因子：
6.6
作者：
Li, Lan;Yang, Longfei;Jiang, Qing
通讯作者：
Jiang, Qing
影响因子：
6.4
作者：
Chu, Constance R.;Szczodry, Michal;Bruno, Stephen
通讯作者：
Bruno, Stephen
影响因子：
5.3
作者：
Di Giancamillo A;Deponti D;Addis A;Domeneghini C;Peretti GM
通讯作者：
Peretti GM
影响因子：
4.8
作者：
Chen, Chen;Song, Jialin;Zhao, Jinzhong
通讯作者：
Zhao, Jinzhong