Raman spectroscopic insight into osteoarthritic cartilage regeneration by mRNA therapeutics encoding cartilage-anabolic transcription factor Runx1.
Raman spectroscopic insight into osteoarthritic cartilage regeneration by mRNA therapeutics encoding cartilage-anabolic transcription factor Runx1.
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DOI：
10.1016/j.mtbio.2022.100210
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发表时间：
2022-01
期刊：
影响因子：
--
通讯作者：
Itaka K
中科院分区：
文献类型：
--
作者：
Pezzotti G;Zhu W;Terai Y;Marin E;Boschetto F;Kawamoto K;Itaka K
虽然关节成形术仍然是目前修复慢性退行性骨关节病最流行的选择，但最近出现了修复受损软骨的可能性，而不是用人工生物材料替代。后一种策略可以避免与全关节置换术相关的侵入性手术。在这个概念的基础上，我们首先应用拉曼光谱来表征内侧副韧带(MCL)和内侧半月板(MM)横断的不稳定诱导性膝骨性关节炎(OA)小鼠的病变软骨。然后，我们通过信使RNA(信使RNA)传递软骨合成矮小相关转录因子1(RUNX1)的方法来检查相同的软骨再生后的骨关节炎模型。拉曼光谱在分子水平上证实了RUNX1mRNA软骨再生方法的治疗效果。这项研究展示了拉曼光谱方法如何支持和加速软骨疾病新疗法的发展。
While joint arthroplasty remains nowadays the most popular option available to repair chronically degenerated osteoarthritic joints, possibilities are recently emerging for regeneration of damaged cartilage rather than its replacement with artificial biomaterials. This latter strategy could allow avoiding the quite intrusive surgical procedures associated with total joint replacement. Building upon this notion, we first apply Raman spectroscopy to characterize diseased cartilage in a mice model of instability-induced knee osteoarthritis (OA) upon medial collateral ligament (MCL) and medial meniscus (MM) transections. Then, we examine the same OA model after cartilage regeneration by means of messenger RNA (mRNA) delivery of a cartilage-anabolic runt-related transcription factor 1 (RUNX1). Raman spectroscopy is shown to substantiate at the molecular scale the therapeutic effect of the Runx1 mRNA cartilage regeneration approach. This study demonstrates how the Raman spectroscopic method could support and accelerate the development of new therapies for cartilage diseases.
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影响因子：
18.2
作者：
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通讯作者：
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通讯作者：
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DOI：
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发表时间：
2008-04-01
影响因子：
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作者：
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通讯作者：
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影响因子：
3.8
作者：
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通讯作者：
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