The effect of DNA polymorphisms and natural variation on crossover hotspot activity in Arabidopsis hybrids.
The effect of DNA polymorphisms and natural variation on crossover hotspot activity in Arabidopsis hybrids.
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DOI：
10.1038/s41467-022-35722-3
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发表时间：
2023-01-03
影响因子：
16.6
通讯作者：
Ziolkowski, Piotr A.
中科院分区：
文献类型：
--
作者：
Szymanska-Lejman, Maja;Dziegielewski, Wojciech;Dluzewska, Julia;Kbiri, Nadia;Bieluszewska, Anna;Poethig, R. Scott;Ziolkowski, Piotr A.
在杂交生物中，基因不同的同源染色体在减数分裂过程中配对和重组；然而，特定类型的多态性对交叉的影响尚不清楚。在这里，为了在拟南芥中分析这一点，我们开发了种子分型方法，该方法可以通过测序对交叉进行大规模并行精细定位。我们表明，在一个生成的区间中观察到的结构变体不会改变交叉频率，除非它们直接位于交叉热点内。自然缺失和cas9诱导缺失都会导致热点活性降低，但不会被邻近热点的增加所补偿。为了研究单核苷酸多态性对交叉形成的影响，我们分析了错配检测缺陷的msh2突变体的热点活性。令人惊讶的是，多态热点在msh2中显示出活性降低。在仅含热点区间为杂合的株系中，交点数高于自交系（纯合）的交点数。我们得出结论，MSH2通过刺激多态区域的热点活动来塑造交叉分布。序列多态性对重组的影响尚不完全清楚。在这里，作者开发了一种种子分型方法，通过测序来绘制杂交（COs），并表明COs优先发生在多态性热点区域，这种效应取决于拟南芥错配修复基因MSH2。
In hybrid organisms, genetically divergent homologous chromosomes pair and recombine during meiosis; however, the effect of specific types of polymorphisms on crossover is poorly understood. Here, to analyze this in Arabidopsis, we develop the seed-typing method that enables the massively parallel fine-mapping of crossovers by sequencing. We show that structural variants, observed in one of the generated intervals, do not change crossover frequency unless they are located directly within crossover hotspots. Both natural and Cas9-induced deletions result in lower hotspot activity but are not compensated by increases in immediately adjacent hotspots. To examine the effect of single nucleotide polymorphisms on crossover formation, we analyze hotspot activity in mismatch detection-deficient msh2 mutants. Surprisingly, polymorphic hotspots show reduced activity in msh2. In lines where only the hotspot-containing interval is heterozygous, crossover numbers increase above those in the inbred (homozygous). We conclude that MSH2 shapes crossover distribution by stimulating hotspot activity at polymorphic regions. The effect of sequence polymorphisms on recombination is not fully understood. Here, the authors develop the seed-typing method to map crossovers (COs) by sequencing and show that COs occur preferentially within polymorphic hotspots and that this effect depends on the mismatch repair gene MSH2 in Arabidopsis.
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影响因子：
14.9
作者：
Ziolkowski PA;Koczyk G;Galganski L;Sadowski J
通讯作者：
Sadowski J
影响因子：
30.8
作者：
Choi, Kyuha;Zhao, Xiaohui;Kelly, Krystyna A.;Venn, Oliver;Higgins, James D.;Yelina, Nataliya E.;Hardcastle, Thomas J.;Ziolkowski, Piotr A.;Copenhaver, Gregory P.;Franklin, F. Chris H.;McVean, Gil;Henderson, Ian R.
通讯作者：
Henderson, Ian R.
ATMUS81在A. thaliana中对干扰不敏感的交叉中的作用。
影响因子：
4.5
作者：
Berchowitz, Luke E.;Francis, Kirk E.;Bey, Alexandra L.;Copenhaver, Gregory P.
通讯作者：
Copenhaver, Gregory P.
影响因子：
4.5
作者：
Choi K;Reinhard C;Serra H;Ziolkowski PA;Underwood CJ;Zhao X;Hardcastle TJ;Yelina NE;Griffin C;Jackson M;Mézard C;McVean G;Copenhaver GP;Henderson IR
通讯作者：
Henderson IR
DOI：
10.15252/embj.2020104858
发表时间：
2020-11-02
期刊：
The EMBO journal
影响因子：
--
作者：
Blackwell AR;Dluzewska J;Szymanska-Lejman M;Desjardins S;Tock AJ;Kbiri N;Lambing C;Lawrence EJ;Bieluszewski T;Rowan B;Higgins JD;Ziolkowski PA;Henderson IR
通讯作者：
Henderson IR