Genome sequence comparison of Col and Ler lines reveals the dynamic nature of Arabidopsis chromosomes.
Genome sequence comparison of Col and Ler lines reveals the dynamic nature of Arabidopsis chromosomes.
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DOI：
10.1093/nar/gkp183
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发表时间：
2009-06
影响因子：
14.9
通讯作者：
Sadowski J
中科院分区：
文献类型：
--
作者：
Ziolkowski PA;Koczyk G;Galganski L;Sadowski J
植物基因组大小的巨大差异主要是由于大量的插入或缺失事件(INDELs)。这些事件之间的平衡决定了基因组变化的进化方向。为了解决什么现象引发这些改变的问题，我们比较了两个拟南芥品系哥伦比亚(Col)和Landsberg erecta(Ler)的基因组序列。根据所得到的比对结果，对这两个基因组中的大Indels(>；100 )进行了分析。这两个基因组之间的差异是由∼8500大Indels组成。其起源的遗传基础可分为三大类：不平等重组(UREC)起源、非法重组(Illrec)起源和转座元件(TE)起源。对它们在染色体上的分布和大小变异的详细研究，以及相关分析，使我们能够展示特定的基于重组的机制对植物基因组进化的影响。结果表明，非均匀复合不能有效地去除着丝粒周围区域的TES。此外，我们发现大Indels对基因进化的影响出人意料地高，指出了不同基因家族之间的显著差异。第一次，我们提出了令人信服的证据，证明体细胞事件在植物基因组进化中确实发挥着重要作用。
Large differences in plant genome sizes are mainly due to numerous events of insertions or deletions (indels). The balance between these events determines the evolutionary direction of genome changes. To address the question of what phenomena trigger these alterations, we compared the genomic sequences of two Arabidopsis thaliana lines, Columbia (Col) and Landsberg erecta (Ler). Based on the resulting alignments large indels (>100 bp) within these two genomes were analysed. There are ∼8500 large indels accounting for the differences between the two genomes. The genetic basis of their origin was distinguished as three main categories: unequal recombination (Urec)-derived, illegitimate recombination (Illrec)-derived and transposable elements (TE)-derived. A detailed study of their distribution and size variation along chromosomes, together with a correlation analyses, allowed us to demonstrate the impact of particular recombination-based mechanisms on the plant genome evolution. The results show that unequal recombination is not efficient in the removal of TEs within the pericentromeric regions. Moreover, we discovered an unexpectedly high influence of large indels on gene evolution pointing out significant differences between the various gene families. For the first time, we present convincing evidence that somatic events do play an important role in plant genome evolution.
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影响因子：
1.7
作者：
Jurka, J;Kapitonov, VV;Walichiewicz, J
通讯作者：
Walichiewicz, J
DOI：
10.1073/pnas.0510791103
发表时间：
2006-03-28
影响因子：
11.1
作者：
Lysak, MA;Berr, A;Schubert, I
通讯作者：
Schubert, I
影响因子：
4.1
作者：
Hosouchi, T;Kumekawa, N;Kotani, H
通讯作者：
Kotani, H
影响因子：
3.1
作者：
Babula, D;Kaczmarek, M;Sadowski, J
通讯作者：
Sadowski, J
影响因子：
7.4
作者：
Boivin, K;Acarkan, A;Schmidt, R
通讯作者：
Schmidt, R