Fast bound pool fraction imaging of the in vivo rat brain: association with myelin content and validation in the C6 glioma model.
Fast bound pool fraction imaging of the in vivo rat brain: association with myelin content and validation in the C6 glioma model.
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DOI：
10.1016/j.neuroimage.2010.10.065
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发表时间：
2011-02-01
期刊：
影响因子：
5.7
通讯作者：
Yarnykh VL
中科院分区：
文献类型：
--
作者：
Underhill HR;Rostomily RC;Mikheev AM;Yuan C;Yarnykh VL
交叉弛豫成像 (CRI) 是一种定量磁共振技术，可测量与水结合的质子和与大分子结合的质子之间磁化传递的动力学参数。在本研究中，首先将 3.0 T 下正常大鼠大脑 (N=5) 的体内四参数 CRI 直接与组织学进行比较。结合池分数 f 与髓磷脂密度密切相关（Pearson’s r = 0.99，p <0.001）。这种相关性在灰质（GM；r = 0.89，p = 0.046）和白质（WM；r = 0.97，p = 0.029）的单独分析中仍然存在。随后，提出了一种新的省时方法，用于仅捕获 f 的全脑参数图，并通过组织学进行验证，并用于估计髓磷脂密度。由于所描述的快速获取 f 的方法对其他 CRI 参数施加了限制，因此对误差进行了理论分析。正常和病理组织中 f 的估计误差预计小于 10%。从 CRI 数据的传统四参数拟合获得的 f 值与建议的 f 快速获取值的比较在体内大鼠脑胶质瘤的预期范围内（N = 4；平均值±SE；分别为 3.9 ± 0.2% 与 4.0 ± 0.2%）。在全脑 f 图和髓磷脂密度图中，可以很容易地识别出增殖和入侵的肿瘤细胞对正常 GM 和 WM 的替代。快速、全脑采集结合池部分可能为动物和人类成像期间检测 GM 和 WM 中的神经胶质瘤侵袭提供可靠的方法。
Cross-relaxation imaging (CRI) is a quantitative magnetic resonance technique that measures the kinetic parameters of magnetization transfer between protons bound to water and protons bound to macromolecules. In this study, in vivo, four-parameter CRI of normal rat brains (N=5) at 3.0 T was first directly compared to histology. The bound pool fraction, f, was strongly associated with myelin density (Pearson’s r = 0.99, p <0.001). The correlation persisted in separate analyses of gray matter (GM; r = 0.89, p =0.046) and white matter (WM; r = 0.97, p =0.029). Subsequently, a new time-efficient approach for solely capturing the whole-brain parametric map of f was proposed, validated with histology, and used to estimate myelin density. Since the described approach for the rapid acquisition of f applied constraints to other CRI parameters, a theoretical analysis of error was performed. Estimates of f in normal and pathologic tissue were expected to have <10% error. A comparison of values for f obtained from the traditional four-parameter fit of CRI data versus the proposed rapid acquisition of f was within this expected margin for in vivo rat brain gliomas (N=4; mean ± SE; 3.9 ± 0.2% vs. 4.0 ± 0.2%, respectively). In both whole-brain f maps and myelin density maps, replacement of normal GM and WM by proliferating and invading tumor cells could be readily identified. The rapid, whole-brain acquisition of the bound pool fraction may provide a reliable method for detection of glioma invasion in both GM and WM during animal and human imaging.
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影响因子：
3.3
作者：
Bjarnason, TA;Vavasour, IM;MacKay, AL
通讯作者：
MacKay, AL
影响因子：
19.7
作者：
BOORSTEIN, JM;WONG, KT;MCGOWAN, JC
通讯作者：
MCGOWAN, JC
影响因子：
3.3
作者：
GRAD, J;MENDELSON, D;BRYANT, RG
通讯作者：
BRYANT, RG
影响因子：
4.1
作者：
Laws, ER;Parney, IF;Chang, S
通讯作者：
Chang, S
影响因子：
3.3
作者：
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通讯作者：
Bieri, O.