A neutralizing monoclonal antibody binds to an epitope near the amino terminus of murine granulocyte-macrophage colony-stimulating factor.
A neutralizing monoclonal antibody binds to an epitope near the amino terminus of murine granulocyte-macrophage colony-stimulating factor.
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中和单克隆抗体与鼠粒细胞-巨噬细胞集落刺激因子氨基末端附近的表位结合。
DOI：
10.1089/hyb.1991.10.433
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Prystowsky,MB
中科院分区：
文献类型：
--
作者：
Meropol,NJ;Kreider,BL;Lee,VM;Kaushansky,K;Prystowsky,MB
A rat anti-murine granulocyte-macrophage colony-stimulating factor (mGM-CSF) monoclonal antibody, A2, that neutralizes bioactivityin vitrowas isolated. The binding epitope recognized by this antibody was identified using human-murine hybrid GM-CSF proteins. A2 was unable to immunoprecipitate a hybrid (hm7) protein containing the human GM-CSF sequence for the first 11 amino terminal amino acids, and the mGM-CSF sequence for amino acids 12-124. In contrast, A2 did recognize a hybrid which substitutes human GM-CSF amino acids 23-36 in the murine sequence. These data suggest that this neutralizing antibody recognizes an epitope at the amino terminus of mGM-CSF. Because hm7 did maintainin vitrobioactivity, it is probable that the epitope recognized by the neutralizing antibody is not itself part of the receptor-binding domain of mGM-CSF; rather, it is likely that neutralization occurs as a result of antibody binding near the receptor-binding site, with steric inhibition of mGM-CSF binding to its receptor. Interestingly, monoclonal antibody A2 does not recognize mGM-CSF glycosylation species corresponding to predicted maximal O-glycosylation variants. The presence of O-glycosylation sites within the antibody-binding epitope was confirmed using site-directed mutagenesis. Potential O-glycosylation sites in native mGM-CSF were removed by introducing conservative amino acid substitutions, and expected molecular weight reductions were obtained. These findings are consistent with previous reports that suggest the importance of the integrity of residues near the amino terminus to GM-CSF bioactivity.
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小鼠粒细胞巨噬细胞集落刺激因子羧基末端残基的缺失会导致生物活性丧失和糖基化改变。
影响因子：
3.9
作者：
LaBranche,CC;Clark,SC;Johnson,GD;Ornstein,D;Sabath,DE;Tushinski,R;Paetkau,V;Prystowsky,MB
通讯作者：
Prystowsky,MB
人转化生长因子 TGF-β2 的瞬时产生和分泌
影响因子：
3.5
作者：
M. Caltabiano;M. Tsang;J. Weatherbee;R. Lucas;G. Sathe;J. Sutton;G. Johnson;D. Bergsma
通讯作者：
D. Bergsma
影响因子：
14.9
作者：
LUTHMAN, H;MAGNUSSON, G
通讯作者：
MAGNUSSON, G
小鼠粒细胞/巨噬细胞集落刺激因子的诱变揭示了 N 末端附近的关键残基。
DOI：
10.1111/j.1432-1033.1987.tb13619.x
发表时间：
1987
期刊：
European journal of biochemistry
影响因子：
--
作者：
Gough,NM;Grail,D;Gearing,DP;Metcalf,D
通讯作者：
Metcalf,D
对 191-194 毫米的蛋白质和肽进行光度分析。
影响因子：
2.9
作者：
M. Mayer;J. Miller
通讯作者：
J. Miller