Monitoring response to radiotherapy in human squamous cell cancer bearing nude mice: comparison of 2'-deoxy-2'-[18F]fluoro-D-glucose (FDG) and 3'-[18F]fluoro-3'-deoxythymidine (FLT).
Monitoring response to radiotherapy in human squamous cell cancer bearing nude mice: comparison of 2'-deoxy-2'-[18F]fluoro-D-glucose (FDG) and 3'-[18F]fluoro-3'-deoxythymidine (FLT).
复制标题
DOI：
10.1007/s11307-007-0104-5
复制
发表时间：
2007-11
影响因子：
3.1
通讯作者：
Lammertsma, Adriaan A.
中科院分区：
文献类型：
--
作者：
Molthoff, Carla F. M.;Klabbers, Bianca M.;Berkhof, Johannes;Felten, Jasper T.;van Gelder, Marcelle;Windhorst, Albert D.;Slotman, Ben J.;Lammertsma, Adriaan A.
在分次放疗前和放疗期间测量了3′-[18 F]氟-3 ′-脱氧胸苷（FLT）（一种增殖标记物）的摄取，以评价FLT-正电子发射断层扫描（PET）成像与2′-脱氧-2 ′-[18 F]氟-d-葡萄糖（FDG）相比作为肿瘤缓解指标的潜力。使用150-kVp装置对荷有已建立的人头颈异种移植物的裸鼠（HNX-OE; nu/nu小鼠）进行局部照射（3次/周; 22戈伊）。在治疗期间采集多次FDG和FLT-PET扫描。定期确定肿瘤体积，并分析组织中涉及示踪剂吸收的生物标志物。与未治疗的肿瘤相比，两组的肿瘤体积均显着下降（P < 0.01）。对于FDG以及FLT，在第4天观察到保留显著下降。对于FLT，在第12天观察到保留的最显著下降;而对于FDG，这在第4天已经注意到。FDG和FLT的肿瘤与非肿瘤比率（T/NT）的最大下降分别为42 ± 18%和49 ± 16%（平均值± SD）。FLT摄取高于FDG。对于FLT，发现基线和第29天的肿瘤体积与T/NT和ΔT/NT具有统计学显著相关性。所有肿瘤均表达葡萄糖转运蛋白-1、胸苷激酶-1和己糖激酶II。治疗结束时，肿瘤细胞数量和坏死率无差异。这种新的实验性体内模型支持使用FLT-PET的承诺，与FDG-PET一样，监测对外部放射治疗的反应。这需要进一步的临床研究来比较这两种示踪剂，特别是在用放射疗法治疗的癌症中。
The uptake of 3′-[18F]fluoro-3′-deoxythymidine (FLT), a proliferation marker, was measured before and during fractionated radiotherapy to evaluate the potential of FLT-positron emission tomography (PET) imaging as an indicator of tumor response compared to 2′-deoxy-2′-[18F]fluoro-d-glucose (FDG). Nude mice bearing established human head and neck xenografts (HNX-OE; nu/nu mice) were locally irradiated (three fractions/week; 22 Gy) using a 150-kVp unit. Multiple FDG- and FLT-PET scans were acquired during treatment. Tumor volume was determined regularly, and tissue was analyzed for biomarkers involved in tracer uptake. Both groups revealed a significant decline in tumor volume (P < 0.01) compared to untreated tumors. For FDG as well as for FLT, a significant decline in retention was observed at day 4. For FLT, most significant decline in retention was observed at day 12; whereas, for FDG, this was already noted at day 4. Maximum decline in tumor-to-nontumor ratios (T/NT) for FDG and FLT was 42 ± 18% and 49 ± 16% (mean ± SD), respectively. FLT uptake was higher then that of FDG. For FLT, statistical significant correlations were found for both tumor volume at baseline and at day 29 with T/NT and ΔT/NT. All tumors demonstrated expression of glucose transporter-1, thymidine kinase-1, and hexokinase II. No differences were found for amount of tumor cells and necrosis at the end of treatment. This new experimental in vivo model supports the promise of using FLT-PET, as with FDG-PET, to monitor response to external radiotherapy. This warrants further clinical studies to compare these two tracers especially in cancers treated with radiotherapy.
登录
查看更多内容
影响因子：
3.1
作者：
Brun, E;Ohlsson, T;Strand, SE
通讯作者：
Strand, SE
影响因子：
4.1
作者：
PATEL, PN;MEHAIA, MA;CHERYAN, M
通讯作者：
CHERYAN, M
影响因子：
2.6
作者：
Hardisson, D
通讯作者：
Hardisson, D
影响因子：
3.1
作者：
Klabbers, Bianca M;Lammertsma, Adriaan A;Slotman, Ben J
通讯作者：
Slotman, Ben J
影响因子：
1.6
作者：
Machulla, HJ;Blocher, A;Grierson, JR
通讯作者：
Grierson, JR