Absorption Mode Fourier Transform Ion Mobility Mass Spectrometry Multiplexing Combined with Half-Window Apodization Windows Improves Resolution and Shortens Acquisition Times.
Absorption Mode Fourier Transform Ion Mobility Mass Spectrometry Multiplexing Combined with Half-Window Apodization Windows Improves Resolution and Shortens Acquisition Times.
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DOI：
10.1021/acs.analchem.1c01427
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发表时间：
2021-07-13
影响因子：
7.4
通讯作者：
Brodbelt, Jennifer S.
中科院分区：
文献类型：
--
作者：
Sanders, James D.;Butalewicz, Jamie P.;Clowers, Brian H.;Brodbelt, Jennifer S.
傅立叶变换多路复用使得能够将漂移管离子迁移率耦合到广泛的质谱仪阵列，与双门信号平均方法相比，具有改进的离子利用率和占空比。传统上，由该方法生成的数据以幅度模式呈现，但是如果数据可以被相位校正并且以吸收模式呈现，则期望分辨率和信噪比（SNR）的显著改善。一种简单可靠地确定和校正傅里叶变换离子迁移率质谱数据中的相移的方法，该方法使用任何用户都容易获得的信息，并在不修改仪器硬件或实验程序的情况下对小分子和完整蛋白质分析进行评估。此外，两种处理方法的切趾和零填充的影响进行评估，并提出和评估使用这些技术来减少采集时间的策略。分辨率提高了1.6的平均因子，信噪比提高了1.2的平均因子，和采集时间减少了80%，通过应用吸收模式处理结合切趾和零填充。
Fourier transform multiplexing enables the coupling of drift tube ion mobility to a wide array of mass spectrometers with improved ion utilization and duty cycles compared to dual-gate signal averaging methods. Traditionally, the data generated by this method is presented in the magnitude mode, but significant improvements in resolution and the signal-to-noise ratio (SNR) are expected if the data can be phase corrected and presented in the absorption mode. A method to simply and reliably determine and correct phase shifts in Fourier transform ion mobility mass spectrometry data using information readily available to any user is presented and evaluated for both small molecule and intact protein analyses with no modification to instrument hardware or experimental procedures. Additionally, the effects of apodization and zero padding are evaluated for both processing methods, and a strategy to use these techniques to reduce acquisition times is presented and evaluated. Resolution is improved by an average factor of 1.6, the SNR is improved by an average factor of 1.2, and acquisition times are reduced by up to 80% through the application of absorption mode processing combined with apodization and zero padding.
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