Effects of photoperiod, hypophysectomy, and follicle-stimulating hormone on testicular follicle-stimulating hormone binding sites in golden hamsters.
Effects of photoperiod, hypophysectomy, and follicle-stimulating hormone on testicular follicle-stimulating hormone binding sites in golden hamsters.
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光周期、垂体切除术和卵泡刺激素对金黄仓鼠睾丸卵泡刺激素结合位点的影响。
DOI：
10.1095/biolreprod37.2.356
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发表时间：
1987
影响因子：
3.6
通讯作者：
Chandrashekar,V
中科院分区：
文献类型：
--
作者：
Klemcke,HG;VanSickle,M;Bartke,A;Amador,A;Chandrashekar,V
本实验部分研究了成年金黄地鼠睾丸中未被占据的卵泡刺激素（FSH）结合位点的调控。使用碘化牛FSH在1800 × gav全睾丸匀浆中测量睾丸FSH结合位点。FSH的结合对FSH具有高度特异性，主要位于睾丸，具有时间和温度依赖性，最初可逆，可饱和，并且与由单一类别的高亲和力结合位点组成的模型一致（平衡结合常数（Ka）范围为2-12 × 1010 M −1）。仓鼠暴露于由5L：19 D组成的短光周期与睾丸FSH结合位点的浓度（fmol/mg蛋白质）增加相关，但总含量（fmol/睾丸）减少。受体亲和力没有明显的5L：19 D相关改变（平均Ka= 7.83 × 1010 M −1）。将绵羊催乳素（oPRL）、绵羊促黄体生成素（oLH）或绵羊促卵泡激素（oFSH）连续3天注射到5L：19 D饲养12周的仓鼠体内，对光周期诱导的睾丸FSH结合位点变化没有影响。在垂体切除术后第5天和第6天，FSH结合位点浓度急剧增加，但对结合位点亲和力的影响很小。垂体切除术后第2、3和4天注射5 µg oFSH可防止第5天测量的结合位点浓度增加。联合注射5 μg oFSH、50 μg oPRL和25 μg oLH也可降低垂体切除仓鼠睾丸FSH结合位点的浓度，但单独注射oPRL或oLH无效。这些数据表明睾丸FSH结合位点的同源下调，但不排除其他激素的参与。
Experiments were conducted to partially characterize and to examine the regulation of unoccupied testicular follicle-stimulating hormone (FSH) binding sites in adult golden hamsters. Testicular FSH binding sites were measured in the 1800 × gavfraction of whole testicular homogenates using iodinated bovine FSH. Binding of FSH was highly specific for FSH, located primarily in the testes, was time- and temperature-dependent, initially reversible, saturable, and consistent with a model consisting of a single class of high-affinity binding sites (range of equilibrium association constants (Ka) 2–12 × 1010M−1). Exposure of hamsters to a short photoperiod consisting of 5L:19D was associated with an increase in concentration (fmol/mg protein), but a reduction in total content (fmol/testes) of testicular FSH binding sites. There was no appreciable 5L:19D-associated alteration in receptor affinity (average Ka= 7.83 × 1010M−1). Injections of ovine prolactin (oPRL), ovine luteinizing hormone (oLH), or ovine FSH (oFSH) for 3 days into hamsters housed in 5L:19D for 12 wk had no effect on photoperiod-induced changes in testicular FSH binding sites. On Days 5 and 6 post hypophysectomy, a dramatic increase in FSH binding site concentration occurred, with but marginal effects on binding site affinity. Injections of 5 µg oFSH on Days 2, 3, and 4 after hypophysectomy prevented the increase in binding site concentrations measured on Day 5. Injection of a combination of 5 µg oFSH, 50 µg oPRL, and 25 µg oLH also reduced testicular FSH binding site concentrations in hypophysectomized hamsters, but oPRL or oLH by themselves were ineffective. The data indicate a homologous down-regulation of testicular FSH binding sites, but do not exclude the involvement of other hormones.
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