Transcriptomic evidence that longevity of acquired plastids in the photosynthetic slugs Elysia timida and Plakobranchus ocellatus does not entail lateral transfer of algal nuclear genes.
Transcriptomic evidence that longevity of acquired plastids in the photosynthetic slugs Elysia timida and Plakobranchus ocellatus does not entail lateral transfer of algal nuclear genes.
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DOI：
10.1093/molbev/msq239
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发表时间：
2011-01
影响因子：
10.7
通讯作者：
Martin W
中科院分区：
文献类型：
--
作者：
Wägele H;Deusch O;Händeler K;Martin R;Schmitt V;Christa G;Pinzger B;Gould SB;Dagan T;Klussmann-Kolb A;Martin W
Sacoglossan海蛞蝓在动物界是独一无二的，因为它们可以隔离并维持从它们赖以为生的虹吸管藻类中获得的活性质体，使动物进行光合作用。尽管大多数棘舌动物会在数小时内消化它们新摄取的质体，但有四种斑鳃动物会在数周到数月的时间内将它们偷来的质体保持在光合作用活跃的状态。这些光合后生动物消化腺细胞细胞质内质体维持的分子基础尚不清楚，但基于先前对单基因的研究，人们普遍认为涉及从藻类食物来源到蛞蝓的基因转移。事实上，正常的质体发育需要数百种核编码蛋白，光系统II中的蛋白质周转在各种条件下都是快速的。此外，动物在进食过程中只隔离藻质体，而不隔离藻核。如果藻类核基因在饲养过程中或在种系中被转移到动物身上，如果它们被表达，那么它们应该很容易用深度测序方法检测到。我们对两种光合作用的海蛞蝓（Plakobranchus ocellatus Van Hasselt， 1824年和Elysia timida Risso， 1818年）积极光合作用的饥饿个体的mrna表达进行了测序。我们发现，核编码的藻类衍生的光合功能特异性基因在P. ocellatus和E. timida中都没有表达。尽管它们具有惊人的质体寿命，但这些光合作用的糖舌蛞蝓不表达从藻核获得的基因，以维持质体功能。
Sacoglossan sea slugs are unique in the animal kingdom in that they sequester and maintain active plastids that they acquire from the siphonaceous algae upon which they feed, making the animals photosynthetic. Although most sacoglossan species digest their freshly ingested plastids within hours, four species from the family Plakobranchidae retain their stolen plastids (kleptoplasts) in a photosynthetically active state on timescales of weeks to months. The molecular basis of plastid maintenance within the cytosol of digestive gland cells in these photosynthetic metazoans is yet unknown but is widely thought to involve gene transfer from the algal food source to the slugs based upon previous investigations of single genes. Indeed, normal plastid development requires hundreds of nuclear-encoded proteins, with protein turnover in photosystem II in particular known to be rapid under various conditions. Moreover, only algal plastids, not the algal nuclei, are sequestered by the animals during feeding. If algal nuclear genes are transferred to the animal either during feeding or in the germ line, and if they are expressed, then they should be readily detectable with deep-sequencing methods. We have sequenced expressed mRNAs from actively photosynthesizing, starved individuals of two photosynthetic sea slug species, Plakobranchus ocellatus Van Hasselt, 1824 and Elysia timida Risso, 1818. We find that nuclear-encoded, algal-derived genes specific to photosynthetic function are expressed neither in P. ocellatus nor in E. timida. Despite their dramatic plastid longevity, these photosynthetic sacoglossan slugs do not express genes acquired from algal nuclei in order to maintain plastid function.
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DOI：
10.1078/0944-2006-00036
发表时间：
2001-01-01
期刊：
ZOOLOGY-ANALYSIS OF COMPLEX SYSTEMS
影响因子：
--
作者：
Rumpho, ME;Summer, EJ;Manhart, JR
通讯作者：
Manhart, JR
DOI：
10.1073/pnas.182432999
发表时间：
2002-09-17
影响因子：
11.1
作者：
Martin, W;Rujan, T;Penny, D
通讯作者：
Penny, D
影响因子：
4.5
作者：
Grzymski, J;Schofield, OM;Bernhard, JM
通讯作者：
Bernhard, JM
影响因子：
64.8
作者：
Johnson, Matthew D.;Oldach, David;Stoecker, Diane K.
通讯作者：
Stoecker, Diane K.
影响因子：
14.9
作者：
Pruitt KD;Tatusova T;Maglott DR
通讯作者：
Maglott DR