Dynamic transcription of long non-coding RNA genes during CD4+ T cell development and activation.
Dynamic transcription of long non-coding RNA genes during CD4+ T cell development and activation.
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CD4 T 细胞发育和激活过程中长非编码 RNA 基因的动态转录
DOI：
10.1371/journal.pone.0101588
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Zhang J
中科院分区：
文献类型：
--
作者：
Xia F;Dong F;Yang Y;Huang A;Chen S;Sun D;Xiong S;Zhang J
研究背景长链非编码RNA（longnoncodingRNA，LncRNA）在细胞发育、活化和肿瘤发生等生物学过程中发挥着重要的调控作用。然而，这些LncRNA在CD4+ T细胞的发育和活化中的作用，这是免疫应答的重要组成部分，仍然未知。结果为了预测LncRNA在CD4+ T细胞发育和活化中的功能，首先，我们在微阵列分析中检测了CD4−CD8−（DN）、CD4+CD8+（DP）、CD4 + CD8−和活化的CD4+CD8− T细胞中LncRNA和mRNA的表达谱，并通过真实的实时PCR（qPCR）验证了这些结果。我们发现，在发育转变的每个过程中，数百种LncRNA的表达都发生了显著变化，包括DN转化为DP，DP转化为CD4+CD8−，CD4+CD8−转化为活化的CD4+ T细胞。Kendall距离分析表明，DN、DP、CD4+CD8− T细胞和活化的CD4+ T细胞中LncRNA的表达与这些T细胞中mRNA的表达相关。Blat算法和GO分析表明LncRNA可能在CD4+ T细胞的发育和活化中发挥重要作用。这些作用包括增殖、稳态、成熟、活化、迁移、凋亡和钙离子转运。结论LncRNA在不同发育阶段的CD4+ T细胞中的表达具有明显差异。LncRNA参与CD4+ T细胞发育和活化的关键生物学过程。
Background Recent evidence shows that long non-coding RNA (LncRNA) play important regulatory roles in many biology process, including cell development, activation and oncogenesis. However, the roles of these LncRNAs in the development and activation of CD4+ T cells, which is an important component of immune response, remain unknown. Results To predict the function of LncRNA in the development and activation of CD4+ T cells, first, we examined the expression profiles of LncRNAs and mRNAs in CD4−CD8− (DN), CD4+CD8+ (DP), CD4+CD8−, and activated CD4+CD8− T cells in a microarray analysis and verified these results by real time PCRs (qPCR). We found that the expression of hundreds of LncRNAs significantly changed in each process of developmental transition, including DN into DP, DP into CD4+CD8−, and CD4+CD8− into activated CD4+ T cells. A Kendall distance analysis suggested that the expression of LncRNAs in DN, DP, CD4+CD8− T cells and activated CD4+ T cells were correlated with the expression of mRNAs in these T cells. The Blat algorithm and GO analysis suggested that LncRNAs may exert important roles in the development and activation of CD4+ T cells. These roles included proliferation, homeostasis, maturation, activation, migration, apoptosis and calcium ion transportation. Conclusion The present study found that the expression profiles of LncRNAs in different stages of CD4+ T cells are distinguishable. LncRNAs are involved in the key biological process in CD4+ T cell development and activation.
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DOI：
10.1084/jem.20120994
发表时间：
2012-07-02
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Ma CS;Deenick EK;Batten M;Tangye SG
通讯作者：
Tangye SG
影响因子：
64.8
作者：
通讯作者：
--
影响因子：
21.3
作者：
通讯作者：
--
影响因子：
10.5
作者：
Cabili, Moran N.;Trapnell, Cole;Rinn, John L.
通讯作者：
Rinn, John L.
影响因子：
15.3
作者：
Toes, R E;Ossendorp, F;Offringa, R;Melief, C J
通讯作者：
Melief, C J