An exploratory study of methicillin-resistant Staphylococcus aureus and SCCmec elements obtained from a community setting along the Texas border with Mexico.
An exploratory study of methicillin-resistant Staphylococcus aureus and SCCmec elements obtained from a community setting along the Texas border with Mexico.
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DOI：
10.1007/s00284-009-9544-2
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发表时间：
2010-05
影响因子：
2.6
通讯作者：
Rampersad, Joanne
中科院分区：
文献类型：
--
作者：
Ammons, David R.;Puttagunta, Raghuveer;Granados, Jose C.;de la Garza, Georgina;Eyambe, George S.;Rampersad, Joanne
对德克萨斯州南部墨西哥边境沿着的耐甲氧西林金黄色葡萄球菌（MRSA）和细菌中的SCCmec元素进行了探索性研究。在2008年9月至12月期间，375个前鼻孔拭子由参加德克萨斯大学泛美分校（UTPA）的学生自行采集，并进行MRSA培养。保留了57个细菌分离株用于进一步分析，其中包括疑似MRSA和其他含SCCmec的细菌。使用PCR检查分离株是否存在nuc、mecA、lukS-PV和spa基因。还进行SCCmec和spa分型。七个S发现了金黄色葡萄球菌分离株，其中6个被分类为MRSA。SCCmec分型结果显示，6株MRSA中有5株为IV型，1株为IV型，大多数为非S.金黄色葡萄球菌菌株是不可分型的，产生的结果表明混合SCCmec类型。六种MRSA菌株中有五种含有已知的spa类型（其中两种对应于USA 300，一种对应于USA 600），而一种菌株具有新的spa类型。只有一个分离株（USA 300 MRSA）对lukS-PV呈阳性。得克萨斯州边境社区在墨西哥很容易获得抗生素而无需处方，并且MRSA和非S之间的SCCmec类型有很强的区分。金黄色葡萄球菌的研究表明，德克萨斯州的这一边境地区可能是唯一适合研究新出现的SCCmec类型、它们的水平转移以及细菌抗生素耐药性的其他方面的地区。
An exploratory study of methicillin-resistant Staphylococcus aureus (MRSA) and SCCmec elements in bacteria along the Mexican border of south Texas was performed. Between September and December of 2008, 375 swabs of anterior nares were self-collected by students attending the University of Texas-Pan American (UTPA) and cultured for MRSA. Fifty seven bacterial isolates were kept for further analysis that included suspected MRSA and other SCCmec-containing bacteria. Isolates were examined for the presence of nuc, mecA, lukS-PV, and spa genes using PCR. SCCmec and spa typing were also performed. Seven S. aureus isolates were found of which six were classified as MRSA. SCCmec typing showed five of the six MRSA strains to be type IV, while one MRSA strain, and most of the non-S. aureus strains, were untypeable, producing results that were indicative of mixed SCCmec types. Five of the six MRSA strains contained known spa types (two of which corresponded to USA300 and one to USA600), while one strain had a novel spa type. Only one isolate, a USA300 MRSA, was positive for lukS-PV. Easy access by the Texas border community to antibiotics in Mexico without a prescription, and the strong partition in SCCmec types between MRSA and non-S. aureus bacteria suggest that this border region of Texas may be uniquely suited for the study of emerging SCCmec types, their horizontal transfer, and perhaps other aspects of antibiotic resistance in bacteria.
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DOI：
10.1016/j.bbrc.2008.10.009
发表时间：
2008-12-19
影响因子：
3.1
作者：
Higuchi, Wataru;Takano, Tomomi;Yamamoto, Tatsuo
通讯作者：
Yamamoto, Tatsuo
影响因子：
14.2
作者：
Ibrahem, S.;Salmenlinna, S.;Vuopio-Varkila, J.
通讯作者：
Vuopio-Varkila, J.
DOI：
10.1007/s10096-007-0254-z
发表时间：
2007-02-01
影响因子：
4.5
作者：
Reischl, U.;Tuohy, M. J.;Linde, H.
通讯作者：
Linde, H.
影响因子：
12
作者：
Rivera, Jose O.;Ho, Hoi;Ascension Perez-Ruvalcaba, Jose
通讯作者：
Ascension Perez-Ruvalcaba, Jose
影响因子：
4.9
作者：
Oliveira, Duarte C.;Milheirico, Catarina;de Lencastre, Herminia
通讯作者：
de Lencastre, Herminia