Overexpression of a novel gene (Pt2015) endows the commercial diatom Phaeodactylum tricornutum high lipid content and grazing resistance.
Overexpression of a novel gene (Pt2015) endows the commercial diatom Phaeodactylum tricornutum high lipid content and grazing resistance.
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DOI：
10.1186/s13068-022-02221-y
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发表时间：
2022-11-26
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影响因子：
--
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由于其生物活性物质和脂质生产力，海洋硅藻是一种具有商业可行性的物种。对生长速度快、含脂量大的菌种或菌株的分离或基因改造日益受到重视。此外，微型浮游动物污染已成为制约三角藻规模化养殖的主要因素之一，严重影响了三角藻的生长，严重阻碍了三角藻产业化生产的进程。在此，基于我们之前的三角棘球蜂转录组学，我们发现了一个影响三角棘球蜂形态型的新基因（ID: 7202015，以下简称Pt2015）。Pt2015蛋白位于质体中，与外泌体组分的部分序列高度同源。使用CRISPR/Cas9方法检测到的Pt2015敲除菌株（称为2015KO）的形态为梭形，而Pt2015过表达菌株（称为oeT）的大多数形态为三辐射型（约95%），这种形态稳定，已经培养了200多代。此外，oeT菌株的生长速度与WT相似，同时积累了更大的脂滴，比WT增加了约30%。更重要的是，在oeT菌株中培养的变形虫的掠食率比在WT中培养的明显降低，这表明oeT可以有效地避免被浮游微动物吃掉。因此，oeT菌株不仅提高了我们对硅藻形态转化的认识，而且在三角藻的大规模培养中具有潜在的应用前景。在线版本包含补充材料，可在10.1186/s13068-022-02221-y获得。
The marine diatom Phaeodactylum tricornutum is a commercially viable species due to its bioactive substances and lipid productivity. Increasing attention has been paid to the isolation or genetic modification of species or strains with a rapid growth rate and large quantities of lipids. Furthermore, contamination of microzooplankton has been one of the major constraints in P. tricornutum large-scale cultivation, which adversely affects growth and greatly impedes the course of biomass production industrialization. Here, based on our previous transcriptomics of P. tricornutum, we found a novel gene (ID: 7202015, hereafter called Pt2015) which affects morphotype of P. tricornutum. Pt2015 protein is located in the plastid, which is highly homologous to part of the sequences of exosome component. The morphotype of the Pt2015 knockout strain (termed 2015KO) using CRISPR/Cas9 method is fusiform, but the Pt2015 overexpression strain (termed oeT) demonstrates a majority triradiate morphotype (approximately 95%) which is stable and has been cultured for more than 200 generations. In addition, the oeT strain demonstrated a similar growth rate to the WT and simultaneously accumulated larger lipids droplets that increased by approximately 30% compared to that of the WT. More importantly, the grazing rate of the amoebae cultured in the oeT strain significantly decreased in comparison with that cultured in WT, suggesting that the oeT can effectively avoid being eaten by microzooplankton. Therefore, the oeT strain not only improves our understanding of morphotype conversion in diatoms but also demonstrates potential applications for large-scale cultivation of P. tricornutum. The online version contains supplementary material available at 10.1186/s13068-022-02221-y.
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影响因子：
5.6
作者：
Galas L;Burel C;Schapman D;Ropitaux M;Bernard S;Bénard M;Bardor M
通讯作者：
Bardor M
影响因子：
16.6
作者：
Karas, Bogumil J.;Diner, Rachel E.;Lefebvre, Stephane C.;McQuaid, Jeff;Phillips, Alex P. R.;Noddings, Chari M.;Brunson, John K.;Valas, Ruben E.;Deerinck, Thomas J.;Jablanovic, Jelena;Gillard, Jeroen T. F.;Beeri, Karen;Ellisman, Mark H.;Glass, John I.;Hutchison, Clyde A., III;Smith, Hamilton O.;Venter, J. Craig;Allen, Andrew E.;Dupont, Christopher L.;Weyman, Philip D.
通讯作者：
Weyman, Philip D.
影响因子：
7.4
作者：
Abida, Heni;Dolch, Lina-Juana;Marechal, Eric
通讯作者：
Marechal, Eric
DOI：
10.1016/j.algal.2017.08.024
发表时间：
2017-11-01
影响因子：
5.1
作者：
Day, John G.;Gong, Yingchun;Hu, Qiang
通讯作者：
Hu, Qiang
影响因子：
5.8
作者：
Holm, Eric R.;Stamper, David M.;Burkholder, JoAnn M.
通讯作者：
Burkholder, JoAnn M.