Transcriptome Sequencing Analysis of Birch (Betula platyphylla Sukaczev) under Low-Temperature Stress
Transcriptome Sequencing Analysis of Birch (Betula platyphylla Sukaczev) under Low-Temperature Stress
复制标题
低温胁迫下桦树（Betula platyphylla Sukaczev）的转录组测序分析
作者：
Siyu Yan;Dawei Zhang;Song Chen;Su Chen
低温是在这项研究中不利地影响植物的生长和发育的常见非生物应力之一。在低温下，低温（6°C）分别为0、1、1.5、2、2.5和3小时。压力。基因本体（GO）和基因组的京都百科全书（KEGG）的功能富集分析是针对不同表达的基因（DEGS）进行的。在“生物学过程”类别下；在“细胞器”和“细胞成分”下，细胞过程，“代谢过程”和“刺激反应”，在“细胞器”类别下在“分子功能”下，“细胞成分”和“催化活性”和“粘附”。 ，例如脂质代谢和苯基丙烷生物合成途径；在低温应力下，转录因子（TF）的表达不同，包括AP2/ERF，C2H2，Myb-Hb样，WRKY，BHLH，WD40类似于GENE BPEV01.C0480.G0081 ），BPEV01.C1074.G0005（钙调蛋白样CML25），bpev01.c1074.g0001（钙结合ef手蛋白）， BPEV01.C2029.G0005（钙调蛋白样蛋白），BPEV01.C0154.G0008（POD），BPEV01.C0015.G0143（N-乙酰基-1-谷氨酸合酶）和BPEV01.C0148.G0010（BPEV01.C0148.G0010）在低温应力下，在高水平上被上调。
Low temperature is one of the common abiotic stresses that adversely affect the growth and development of plants. In this study, we used RNA-Seq to identify low-temperature-responsive genes in birch and further analyzed the underlying molecular mechanism. Birch seedlings were treated by the low temperature (6 °C) for 0, 1, 1.5, 2, 2.5, and 3 h, respectively. A total of 3491 genes were differentially expressed after low-temperature stress. Gene Ontology (GO) and Kyoto Encyclopedia of Gene and Genomes (KEGG) functional enrichment analysis were performed for the differentially expressed genes (DEGs). GO analysis indicated that 3491 DEGs were distributed into 1002 categories, and these DEGs were enriched in “cell process”, “metabolic process”, and “stimulus response”, under the “biological process” category; in “organelles” and “cell components”, under the “cell component” category; and in “catalytic activity” and “adhesion”, under the “molecular function” category. The KEGG enrichment indicated that 119 DEGs were involved in Ca2+ and plant hormone signal transduction; 205 DEGs were involved in secondary metabolic processes, such as lipid metabolism and phenylpropanoid biosynthesis pathway; and 20 DEGs were involved in photosynthesis. In addition, a total of 362 transcription factors (TFs) were differentially expressed under low-temperature stress, including AP2/ERF, C2H2, MYB-HB-like, WRKY, bHLH, WD40-like, and GRAS families. Gene Bpev01.c0480.g0081 (calmodulin-like CML38), Bpev01.c1074.g0005 (calmodulin-like CML25), Bpev01.c1074.g0001 (Calcium-binding EF-hand family protein), Bpev01.c2029.g0005 (calmodulin-like protein), Bpev01.c0154.g0008 (POD), Bpev01.c0015.g0143 (N-acetyl-1-glutamate synthase), and Bpev01.c0148.g0010 (branched chain amino acid transferase) were up-regulated at a high level, under low-temperature stress.
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影响因子：
4.4
作者：
Wang XC;Zhao QY;Ma CL;Zhang ZH;Cao HL;Kong YM;Yue C;Hao XY;Chen L;Ma JQ;Jin JQ;Li X;Yang YJ
通讯作者：
Yang YJ
影响因子：
5.6
作者：
Kurepin LV;Dahal KP;Savitch LV;Singh J;Bode R;Ivanov AG;Hurry V;Hüner NP
通讯作者：
Hüner NP
垂枝桦 NAC 基因家族的全基因组分析
影响因子：
2.9
作者：
Chen, Song;Lin, Xin;Chen, Su
通讯作者：
Chen, Su
影响因子：
1.8
作者：
A. Michalak
通讯作者：
A. Michalak
影响因子：
3.7
作者：
Sharma R;Singh G;Bhattacharya S;Singh A
通讯作者：
Singh A