Keeping It Clean: The Cell Culture Quality Control Experience at the National Center for Advancing Translational Sciences.
Keeping It Clean: The Cell Culture Quality Control Experience at the National Center for Advancing Translational Sciences.
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DOI：
10.1177/2472555220911451
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发表时间：
2020-06
期刊：
影响因子：
--
通讯作者：
Hall MD
中科院分区：
文献类型：
--
作者：
Roth JS;Lee TD;Cheff DM;Gosztyla ML;Asawa RR;Danchik C;Michael S;Simeonov A;Klumpp-Thomas C;Wilson KM;Hall MD
Quality control monitoring of cell lines utilized in biomedical research is of utmost importance and is critical for reproducibility of data. Two key pitfalls in tissue culture are 1) cell line authenticity, and 2) Mycoplasma contamination. As a collaborative research institute, the National Center for Advancing Translational Sciences receives cell lines from a range of commercial and academic sources, which are adapted for high-throughput screening. Here, we describe the implementation of a routine NCATS-wide Mycoplasma testing and short-tandem repeat (STR) testing for cell lines. Initial testing identified a >10% Mycoplasma contamination rate. While the implementation of systematic testing has not fully suppressed Mycoplasma contamination rates, clearly defined protocols that include immediate destruction of contaminated cell lines wherever possible has enabled rapid intervention and removal of compromised cell lines. Data for >2,000 cell line samples tested over three years, and case studies are provided. STR testing of 186 cell lines with established STR profiles revealed only five mis-identified cell lines, all of which were received from external labs. The data collected over the three years since implementation of this systematic testing demonstrates the importance of continual vigilance for rapid identification of ‘problem’ cell lines, for ensuring reproducible data in translational science research.
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