Genetic profiling reveals cross-contamination and misidentification of 6 adenoid cystic carcinoma cell lines: ACC2, ACC3, ACCM, ACCNS, ACCS and CAC2.
Genetic profiling reveals cross-contamination and misidentification of 6 adenoid cystic carcinoma cell lines: ACC2, ACC3, ACCM, ACCNS, ACCS and CAC2.
复制标题
DOI：
10.1371/journal.pone.0006040
复制
发表时间：
2009-06-25
期刊：
影响因子：
3.7
通讯作者：
Tetsu O
中科院分区：
文献类型：
--
作者：
Phuchareon J;Ohta Y;Woo JM;Eisele DW;Tetsu O
腺样囊性癌是涎腺第二常见的恶性肿瘤。大多数患者在手术和术后放射治疗后存活超过5年。然而，由于局部复发和远处转移，10年生存率下降到40%。提高ACC的长期生存率需要在更好地了解ACC.Much的生物学行为的基础上开发更有效的全身治疗，这一领域的临床前研究涉及使用培养细胞，迄今为止，已经建立了几种ACC细胞系。然而，这些细胞系的鉴定尚未报道。我们使用短串联重复序列（STR）检测对六个ACC细胞系进行DNA指纹分析，发现所有六个细胞系都被其他细胞污染。ACC2、ACC3和ACCM被确定为宫颈癌细胞（HeLa细胞），而ACCS细胞系由T24膀胱癌细胞组成。ACCNS和CAC2细胞被来自非人哺乳动物物种的细胞污染：标记ACCNS的细胞是小鼠细胞，CAC2细胞是大鼠细胞。这些观察结果表明，未来使用ACC细胞系的研究应包括细胞系认证，以避免使用污染或非人类细胞。
Adenoid cystic carcinoma (ACC) is the second most common malignant neoplasm of the salivary glands. Most patients survive more than 5 years after surgery and postoperative radiation therapy. The 10 year survival rate, however, drops to 40%, due to locoregional recurrences and distant metastases. Improving long-term survival in ACC requires the development of more effective systemic therapies based on a better understanding of the biologic behavior of ACC. Much preclinical research in this field involves the use of cultured cells and, to date, several ACC cell lines have been established. Authentication of these cell lines, however, has not been reported. We performed DNA fingerprint analysis on six ACC cell lines using short tandem repeat (STR) examinations and found that all six cell lines had been contaminated with other cells. ACC2, ACC3, and ACCM were determined to be cervical cancer cells (HeLa cells), whereas the ACCS cell line was composed of T24 urinary bladder cancer cells. ACCNS and CAC2 cells were contaminated with cells derived from non-human mammalian species: the cells labeled ACCNS were mouse cells and the CAC2 cells were rat cells. These observations suggest that future studies using ACC cell lines should include cell line authentication to avoid the use of contaminated or non-human cells.
登录
查看更多内容
DOI：
10.1098/rsbl.2003.0025
发表时间：
2003-08-07
影响因子：
4.7
作者：
Hebert, PDN;Ratnasingham, S;deWaard, JR
通讯作者：
deWaard, JR
影响因子：
5
作者：
Brown, J;Reading, SJ;Brown, CA
通讯作者：
Brown, CA
DOI：
10.1016/s0901-5027(05)80830-6
发表时间：
1997-04-01
影响因子：
2.4
作者：
Guan, XF;Qiu, WL;Zhou, XAJA
通讯作者：
Zhou, XAJA
影响因子：
1.7
作者：
Azari, Shahram;Ahmadi, Nahid;Shokri, Fazel
通讯作者：
Shokri, Fazel
影响因子：
4.8
作者：
França, CM;Jaeger, MMM;Araújo, NS
通讯作者：
Araújo, NS