A gain-of-function mutation of OsMAPK6 leads to long grain in rice

A gain-of-function mutation of OsMAPK6 leads to long grain in rice
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OsMAPK6 的功能获得性突变导致水稻长粒

DOI:
10.1016/j.cj.2021.03.022
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发表时间:
2021-05
期刊:
影响因子:
6.6
通讯作者:
Chuanqing Sun
Chuanqing Sun
中科院分区:
农林科学1区
文献类型:
--
作者:
Lina Xiong;Lubin Tan;Ran Xu;Zuofeng Zhu;Xianyou Sun;Hongying Sun;Chuanqing Sun

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籽粒大小是控制粮食产量的重要农艺性状之一。大粒型新种质的开发将有利于作物的改良。本文报道了与丝裂原激活蛋白激酶6(OsMAPK 6)同源的影响水稻粒长的基因LONG GRAIN 6(LOG 6)的遗传鉴定和功能分析。图位克隆分析表明,log 6-D的长粒表型是由于OsMAPK 6保守的TEY基序中谷氨酰胺(E)突变为赖氨酸(K)所致。在近等基因系中,log 6-D等位基因使桂超2号、特青和93-11的粒长和产量增加。序列分析表明,水稻基因组中存在10种OsMAPK 6单倍型,籼、粳两种单倍型存在差异。本研究为MAPKs的功能研究提供了新的思路,为水稻产量的提高提供了一个新的显性等位基因。
Grain size is one of the most important agronomic traits controlling grain yield. Development of novel germplasm with large grains would be beneficial for crop improvement. We report the genetic identification and functional analysis of theLONG GRAIN 6(LOG6) gene, which is identical toMITOGEN-ACTIVATED PROTEIN KINASE 6(OsMAPK6), affecting grain length of rice. Map-based cloning revealed that the long-grain phenotype oflog6-Dresults from a glutamine (E) to lysine (K) mutation in the conserved TEY motif of OsMAPK6. In near-isogenic lines (NILs), thelog6-Dallele increased grain length and grain yield of Guichao 2 (GC2), Teqing (TQ), and 93–11. Sequence analysis revealed 10OsMAPK6haplotypes, withxian(indica) andgeng(japonica) harboring different haplotypes. Our findings shed light on the function of MAPKs and offer a novel dominant allele for improving the grain yield of rice.
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