A Cryophyte Transcription Factor, CbABF1, Confers Freezing, and Drought Tolerance in Tobacco
A Cryophyte Transcription Factor, CbABF1, Confers Freezing, and Drought Tolerance in Tobacco
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低温植物转录因子 CbABF1 赋予烟草抗冻和干旱能力
DOI：
10.3389/fpls.2019.00699
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发表时间：
2019-05
影响因子：
5.6
通讯作者：
An Lizhe
中科院分区：
文献类型：
--
作者：
Yue Xiule;Zhang Guoyan;Zhao Zhen;Yue Jinli;Pu Xiaohong;Sui Mengjun;Zhan Yi;Shi Yulan;Wang Zhenyu;Meng Guanghua;Zhao Zhixing;An Lizhe
脱落酸反应元件结合因子(ABF)在植物对非生物胁迫的响应中起着至关重要的作用。然而，人们对ABF在高山亚热带植物中的作用知之甚少，这些植物可以在极端环境条件下生存。在这里，我们克隆并鉴定了高山亚热带植物白背天牛的ABF1同源基因CbABF1。CbABF1的表达受寒冷、干旱和脱落酸的诱导。亚细胞定位分析表明，CbABF1定位于细胞核。此外，CbABF1具有反式激活活性，该活性依赖于含有89个残基的N末端区域。在酵母双杂交和双分子荧光互补分析中，Snf1相关蛋白激酶CbSnRK2.6与CbABF1相互作用。瞬时表达分析表明，CbSnRK2.6增强了CbABF1对Abre顺式元件的反式激活作用。我们进一步发现，CbABF1在烟草中的异源表达提高了植物对低温和干旱胁迫的耐受性，其中转基因植株的存活率比野生型植株分别提高了40%和60%左右。此外，转基因植物积累的活性氧较少，同时抗氧化酶活性较高，逆境反应基因的表达增加。我们的结果表明，CbABF1是一个在抗寒、抗旱中起重要作用的转录因子，是耐逆作物分子育种的候选基因。
Abscisic acid responsive element binding factors (ABFs) play crucial roles in plant responses to abiotic stress. However, little is known about the roles of ABFs in alpine subnival plants, which can survive under extreme environmental conditions. Here, we cloned and characterized an ABF1 homolog, CbABF1, from the alpine subnival plant Chorispora bungeana. Expression of CbABF1 was induced by cold, drought, and abscisic acid. Subcellular localization analysis revealed that CbABF1 was located in the nucleus. Further, CbABF1 had transactivation activity, which was dependent on the N-terminal region containing 89 residues. A Snf1-related protein kinase, CbSnRK2.6, interacted with CbABF1 in yeast two-hybrid analysis and bimolecular fluorescence complementation assays. Transient expression assay revealed that CbSnRK2.6 enhanced the transactivation of CbABF1 on ABRE cis-element. We further found that heterologous expression of CbABF1 in tobacco improved plant tolerance to freezing and drought stress, in which the survival rates of the transgenic plants increased around 40 and 60%, respectively, compared with wild-type plants. Moreover, the transgenic plants accumulated less reactive oxygen species, accompanied by high activities of antioxidant enzymes and elevated expression of stress-responsive genes. Our results thus suggest that CbABF1 is a transcription factor that plays an important role in cold and drought tolerance and is a candidate gene in molecular breeding of stress-tolerant crops.
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影响因子：
3.7
作者：
Sirichandra C;Davanture M;Turk BE;Zivy M;Valot B;Leung J;Merlot S
通讯作者：
Merlot S
DOI：
10.1007/s11240-011-0029-1
发表时间：
2011-09
期刊：
Plant Cell, Tissue and Organ Culture (PCTOC)
影响因子：
--
作者：
N. Yang;bullet X L Yue;bullet X L Chen;G. F. Wu;bullet T G Zhang;bullet L Z An
通讯作者：
N. Yang;bullet X L Yue;bullet X L Chen;G. F. Wu;bullet T G Zhang;bullet L Z An
对大豆中铝毒性反应的 MATE 转运蛋白和 MATE 基因亚组的表达模式进行全基因组分析
影响因子：
4.4
作者：
Liu J;Li Y;Wang W;Gai J;Li Y
通讯作者：
Li Y
影响因子：
5.3
作者：
Dong, Chun-Hai;Hu, Xiangyang;Zhu, Jian-Kang
通讯作者：
Zhu, Jian-Kang
影响因子：
4.3
作者：
Lu, Guojun;Gao, Chenxi;Han, Bin
通讯作者：
Han, Bin