CircPVT1 promotes progression in clear cell renal cell carcinoma by sponging miR-145-5p and regulating TBX15 expression.
CircPVT1 promotes progression in clear cell renal cell carcinoma by sponging miR-145-5p and regulating TBX15 expression.
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DOI：
10.1111/cas.14814
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发表时间：
2021-04
期刊：
影响因子：
5.7
通讯作者：
Xie W
中科院分区：
文献类型：
--
作者：
Zheng Z;Chen Z;Zhong Q;Zhu D;Xie Y;Shangguan W;Xie W
Emerging evidence revealed that circular RNAs (circRNAs) play significant roles in regulating tumorigenesis and cancer progression. However, few circRNAs were well characterized in clear cell renal cell carcinoma (ccRCC). We found that circPVT1 was significantly upregulated in ccRCC tissues and positively associated with the clinical stage. The Area Under Curve of tissue and serum circPVT1 expression in ccRCC were 0.93 and 0.86, respectively. Importantly, we demonstrated that circPVT1 promoted ccRCC growth and metastasis in vitro and in vivo. We also found that circPVT1 directly binds to miRNA‐145‐5p via the Biotin‐labelled miRNA pulldown assay and dual‐luciferase reporter assay, and miR‐145‐5p inhibitor significantly attenuated the effect of circPVT1 knockdown on ccRCC cells. Moreover, through RNA sequencing and bioinformatics analysis, we demonstrated that TBX15 was regulated by the circPVT1/miR‐145‐5p axis and predicted poor prognosis in ccRCC. These findings suggest that circPVT1 promotes ccRCC growth and metastasis through sponging miR‐145‐5p and regulating downstream target TBX15 expression. The circPVT1/miR‐145‐5p/TBX15 axis might be a potential diagnostic marker and therapeutic target in ccRCC. We identified an oncogenic circular RNA circPVT1, which was overexpressed in clear cell renal cell carcinoma (ccRCC) tissues and promoted the growth and metastasis of ccRCC in vivo and in vitro. circPVT1 directly bound to miR‐145‐5p and subsequently inhibited its suppressing capability on TBX15. Our study highlighted the regulatory role of the circPVT1/miR‐145‐5p/TBX15 axis in ccRCC and provided a novel potential diagnostic biomarker and therapeutic target for ccRCC patients.
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DOI：
10.1038/nrc.2017.99
发表时间：
2018-01
期刊：
Nature reviews. Cancer
影响因子：
--
作者：
Anastasiadou E;Jacob LS;Slack FJ
通讯作者：
Slack FJ
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影响因子：
14.9
作者：
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通讯作者：
Zhang Z
影响因子：
14.9
作者：
Backes C;Kehl T;Stöckel D;Fehlmann T;Schneider L;Meese E;Lenhof HP;Keller A
通讯作者：
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影响因子：
11.1
作者：
Wei, Siliang;Zheng, Yuanyuan;Li, Wei
通讯作者：
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影响因子：
4.1
作者：
Dudekulay, Dawood B.;Panda, Amaresh C.;Gorospe, Myriam
通讯作者：
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