Regeneration following duplication of imaginal wing disc fragments of Drosophila melanogaster.
Regeneration following duplication of imaginal wing disc fragments of Drosophila melanogaster.
复制标题
果蝇成虫翅盘碎片复制后的再生。
DOI：
10.1016/0012-1606(82)90375-x
复制
发表时间：
1982
影响因子：
2.7
通讯作者：
Schneiderman,HA
中科院分区：
文献类型：
--
作者：
Kirby,BS;Bryant,PJ;Schneiderman,HA
从黑腹果蝇的想象翼盘中取出的碎片在体内培养了28天。一种类型的边缘碎片首先复制，然后停止生长，但其他类型的边缘碎片通常在盘的其他区域的初始复制和再生结构特征之后继续生长。经过28天的培养，大约50%的来自假定的椎间盘腹铰链区的碎片广泛生长，并产生再生和原始结构。有些种植体的再生结构是在镜像对称线上产生的。再生与片段的生长有关，在许多情况下伴随着重复结构的丢失。在某些情况下，只有在培养期后才复制的片段可以通过在新鲜宿主中的额外培养来刺激生长，但在每个宿主中共培养两个片段的结果表明，仅靠培养条件并不能控制片段的生长和调节。大的、正常再生的碎片与腹侧碎片互补，再生后似乎没有生长，只是在长期培养过程中偶尔产生额外的结构。在类似条件下培养的完整翼盘从未产生过多余的结构。我们的结果表明，复制的图案不如完整的再生图案稳定，而完整的再生图案又不如完整的盘稳定。我们认为，镜像对称线上存在的极性反转刺激了复制的磁盘碎片的生长。
Fragments from the imaginal wing disc ofDrosophila melanogasterwere culturedin vivofor periods up to 28 days. One type of edge fragment first duplicated and then ceased to grow, but others often continued to grow following initial duplication and regenerated structures characteristic of other areas of the disc. After 28 days of culture, about 50% of fragments from the presumptive ventral hinge region of the disc grew extensively and produced regenerated as well as original structures. The regenerated structures in some implants were produced at the line of mirror-image symmetry. Regeneration was associated with fragment growth and in many cases was accompanied by loss of duplicate structures. Fragments which were only duplicated after the culture period could in some cases be stimulated to grow by additional culture in fresh hosts, but the results of coculturing two fragments in each host show that culture conditions alone do not control growth and regulation in the fragments. The large, normally regenerating fragment, complementary to the ventral fragment, did not appear to grow following regeneration and only occasionally produced supernumerary structures during prolonged culture. Intact wing discs cultured under similar conditions never produced supernumerary structures. Our results suggest that a duplicated pattern is less stable than a complete, regenerated pattern, which in turn is less stable than an intact disc. We propose that the growth of duplicated disc fragments is stimulated by polarity reversals present at lines of mirror-image symmetry.
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影响因子：
2.7
作者：
SCHUBIGER, G
通讯作者：
SCHUBIGER, G
果蝇原始碎片中的Mustergliederung
影响因子：
2.7
作者：
H. Lüönd
通讯作者：
H. Lüönd
作为果蝇成虫翼盘生长调节因子的位置信息
DOI：
10.1016/b978-0-12-454340-9.50028-8
发表时间：
1980
期刊：
Journal of embryology and experimental morphology
影响因子：
--
作者：
C. Duranceau;S. Glenn;H. Schneiderman
通讯作者：
H. Schneiderman
黑腹果蝇成虫翅和悬垂盘细胞之间的位置特异性相互作用。
影响因子：
2.7
作者：
Paul N. Adler
通讯作者：
Paul N. Adler
影响因子：
2.7
作者：
G. Schubiger;M. Schubiger
通讯作者：
M. Schubiger