Haustoria – arsenals during the interaction between wheat and Puccinia striiformis f. sp. tritici
Haustoria – arsenals during the interaction between wheat and Puccinia striiformis f. sp. tritici
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小麦和条锈菌 f 之间相互作用期间的吸器 - 武库。
DOI：
10.1111/mpp.12882
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发表时间：
2019-11
影响因子：
4.9
通讯作者：
Xiaojie Wang
中科院分区：
文献类型：
--
作者：
Qiang Xu;Chunlei Tang;Likun Wang;Congcong Zhao;Zhensheng Kang;Xiaojie Wang
摘要 作为专性寄生虫，条锈菌 f. sp。小麦（Pst）形成吸器，从植物细胞中获取营养以进行发育，这些结构对于病原体的生存至关重要。为了更好地了解吸器对与寄主植物相互作用的贡献，我们从感染 Pst 种族 CYR31 的易感小麦叶片中分离出吸器，并对它们的转录组以及热孢子和芽管的转录组进行了测序，并比较了三个转录组。从吸器中总共获得了3524个上调基因，其中73个基因与硫胺素生物合成、糖酵解和脂质代谢过程相关。沉默七个基因会降低小麦中 Pst 的生长和发育。更有趣的是，吸器中检测到了 1197 个吸器分泌蛋白（HASP），占总蛋白的 34%，表明这些 HASP 在吸器介导的致病进展中发挥着重要作用。此外，69 个 HASP 能够抑制烟草中 Bax 触发的程序性细胞死亡。此外，46 HASP 使用 III 型分泌系统显着减少了小麦中的胼胝质沉积。该研究通过转录组测序鉴定了大量的效应子，结果揭示了影响病原体生长和定植的代谢途径的组成部分，并表明吸器在 Pst 的生长和致病性中的重要功能。
Summary As an obligate parasite, Puccinia striiformis f. sp. tritici (Pst) forms haustoria to obtain nutrients from plant cells for development, and these structures are essential for pathogen survival. To better understand the contribution of haustoria to the interactions with the host plants, we isolated haustoria from susceptible wheat leaves infected with Pst race CYR31 and sequenced their transcriptome as well as those of urediospores and germ tubes, and compared the three transcriptomes. A total of 3524 up‐regulated genes were obtained from haustoria, of which 73 genes were related to thiamine biosynthesis, glycolysis and lipid metabolic processes. Silencing seven of the genes reduced the growth and development of Pst in wheat. More interestingly, 1197 haustorial secreted proteins (HASPs) were detected in haustoria, accounting for 34% of the total proteins, indicating that these HASPs play important roles in haustorium‐mediated pathogenic progression. Furthermore, 69 HASPs were able to suppress Bax‐triggered programmed cell death in tobacco. Additionally, 46 HASPs significantly reduced callose deposition in wheat using the type III secretion system. This study identified a large number of effectors through transcriptome sequencing, and the results revealed components of metabolic pathways that impact the growth and colonization of the pathogen and indicate essential functions of haustoria in the growth and pathogenicity of Pst.
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影响因子：
4.9
作者：
Jiasui Zhan;Celeste C. Linde;Tim Jurgens;Ueli Merz;Fabrizio Steinebrunner;Bruce A. McDonald
通讯作者：
Jiasui Zhan;Celeste C. Linde;Tim Jurgens;Ueli Merz;Fabrizio Steinebrunner;Bruce A. McDonald
影响因子：
3.7
作者：
Saunders DG;Win J;Cano LM;Szabo LJ;Kamoun S;Raffaele S
通讯作者：
Raffaele S
影响因子：
3.7
作者：
Garnica DP;Upadhyaya NM;Dodds PN;Rathjen JP
通讯作者：
Rathjen JP
了解小麦专性生物营养真菌的生活方式和致病机制：新兴的基因组学时代
DOI：
10.1016/j.cj.2017.11.003
发表时间：
2017-12
期刊：
The Crop Journal
影响因子：
--
作者：
Chunlei Tang;Qiang Xu;Mengxin Zhao;Xiaojie Wang;Zhensheng Kang
通讯作者：
Zhensheng Kang
影响因子：
11.4
作者：
Abramovitch, RB;Kim, YJ;Martin, GB
通讯作者：
Martin, GB