Membrane potential gradient is carbon monoxide-dependent in mouse and human small intestine.
Membrane potential gradient is carbon monoxide-dependent in mouse and human small intestine.
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小鼠和人类小肠中的膜电位梯度依赖于一氧化碳。
DOI：
10.1152/ajpgi.00037.2007
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发表时间：
2007
期刊：
影响因子：
--
通讯作者：
Szurszewski,JosephH
中科院分区：
文献类型：
--
作者：
Sha,Lei;Farrugia,Gianrico;Harmsen,WScott;Szurszewski,JosephH
本研究的目的是量化小鼠和人小肠环形肌层厚度的静息膜电位（RMP）变化，并确定RMP梯度是否依赖于内源性一氧化碳（CO）的产生。用常规的尖锐玻璃微电极记录人小肠和野生型、HO 2-KO和W/WV突变小鼠小肠不同深度的环形平滑肌细胞的RMP。在野生型小鼠和人类肠道中，肌间神经丛附近的环形平滑肌细胞的RMP分别为−65.3 ± 2 mV和−58.4 ± 2 mV，粘膜下边缘的环形平滑肌细胞的RMP分别为−60.1 ± 2 mV和−49.1 ± 1 mV。氧合血红蛋白（20 μM），一氧化碳的捕获剂，和铬中卟啉IX，血红素加氧酶的抑制剂，消除跨壁梯度。NG-硝基-L-精氨酸（200 μM）不改变小鼠和人小肠的RMP梯度。HO 2-KO小鼠和W/WV突变小鼠均未发现跨壁RMP梯度。TTX（1 μM）和1H-[1，2，4-]恶二唑并[4，3-a]喹喔啉-1-酮（10 μM）对RMP梯度无影响。这些数据表明，跨小鼠和人小肠环形肌肉层厚度的RMP梯度是CO依赖性的。
The aims of this study were to quantify the change in resting membrane potential (RMP) across the thickness of the circular muscle layer in the mouse and human small intestine and to determine whether the gradient in RMP is dependent on the endogenous production of carbon monoxide (CO). Conventional sharp glass microelectrodes were used to record the RMPs of circular smooth muscle cells at different depths in the human small intestine and in wild-type, HO2-KO, and W/WVmutant mouse small intestine. In the wild-type mouse and human intestine, the RMP of circular smooth muscle cells near the myenteric plexus was −65.3 ± 2 mV and −58.4 ± 2 mV, respectively, and −60.1 ± 2 mV and −49.1 ± 1 mV, respectively, in circular smooth muscle cells at the submucosal border. Oxyhemoglobin (20 μM), a trapping agent for CO, and chromium mesoporphyrin IX, an inhibitor of heme oxygenase, abolished the transwall gradient. The RMP gradients in mouse and human small intestine were not altered byNG-nitro-l-arginine (200 μM). No transwall RMP gradient was found in HO2-KO mice and W/WVmutant mice. TTX (1 μM) and 1H-[1,2,4-]oxadiazolo[4,3-a]quinoxalin-1-one (10 μM) had no effect on the RMP gradient. These data suggest that the gradient in RMP across the thickness of the circular muscle layer of mouse and human small intestine is CO dependent.
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DOI：
--
发表时间：
1999-10
期刊：
Drug metabolism and disposition: the biological fate of chemicals
影响因子：
--
作者：
Scott D. Appleton;Marc L Chretien;B. McLaughlin;H. Vreman;David K. Stevenson;James F. Brien;Kanji Nakatsu;Donald H. Maurice;Gerald S. Marks
通讯作者：
Scott D. Appleton;Marc L Chretien;B. McLaughlin;H. Vreman;David K. Stevenson;James F. Brien;Kanji Nakatsu;Donald H. Maurice;Gerald S. Marks
DOI：
10.1113/jphysiol.1985.sp015793
发表时间：
1985
期刊：
The Journal of physiology
影响因子：
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作者：
Bauer,AJ;Reed,JB;Sanders,KM
通讯作者：
Sanders,KM
DOI：
10.1152/ajpcell.1988.255.6.c828
发表时间：
1988
期刊：
The American journal of physiology
影响因子：
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作者：
Smith,TK;Reed,JB;Sanders,KM
通讯作者：
Sanders,KM
DOI：
10.1152/ajpheart.00782.2003
发表时间：
2004-02
期刊：
American journal of physiology. Heart and circulatory physiology
影响因子：
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作者：
Qi Xi;Dilyara Tcheranova;H. Parfenova;B. Horowitz;C. Leffler;J. Jaggar
通讯作者：
Qi Xi;Dilyara Tcheranova;H. Parfenova;B. Horowitz;C. Leffler;J. Jaggar
DOI：
10.1073/pnas.92.7.2568
发表时间：
1995-03-28
影响因子：
11.1
作者：
KHARITONOV, VG;SHARMA, VS;KOESLING, D
通讯作者：
KOESLING, D