Time-dependent gene expression analysis after mouse skeletal muscle contusion.
Time-dependent gene expression analysis after mouse skeletal muscle contusion.
复制标题
小鼠骨骼肌挫伤后时间依赖性基因表达分析
DOI：
10.1016/j.jshs.2016.01.017
复制
发表时间：
2016-03
影响因子：
11.7
通讯作者：
Chen P
中科院分区：
文献类型：
--
作者：
Xiao W;Liu Y;Luo B;Zhao L;Liu X;Zeng Z;Chen P
虽然骨骼肌再生的机制已被深入了解，但肌肉挫伤（运动医学诊所中最常见的肌肉损伤之一）所涉及的机制还没有。本研究的目的是探讨肌肉挫伤后再生的机制。在这项研究中，总共使用了72只小鼠。其中8人被随机选择为对照组，而其余的人受到肌肉挫伤。随后，在不同时间点采集他们的腓肠肌。HE染色观察肌肉形态学变化。此外，通过实时聚合酶链反应分析基因表达。数据显示，许多基因的表达，即，免疫细胞和卫星细胞的特异性标志物、肌肉再生的调节因子、细胞因子和趋化因子在恢复的早期阶段增加，特别是在前3天。而且，这些基因的表达有着严格的规则。然而，几乎所有的基因在损伤后14天恢复正常。肌肉挫伤后免疫细胞侵入的顺序为中性粒细胞、M1巨噬细胞和M2巨噬细胞。一些CC（CCL 2，CCL 3和CCL 4）和CXC（CXCL 10）趋化因子可能参与这些免疫细胞的趋化性。HGF可能是肌挫伤后激活卫星细胞的主要因素。此外，如本研究所用，发生急性挫伤时需要2周恢复。
Though the mechanisms of skeletal muscle regeneration are deeply understood, those involved in muscle contusion, one of the most common muscle injuries in sports medicine clinics, are not. The objective of this study is to explore the mechanisms involved in muscle regeneration after contusion injury. In this study, a total of 72 mice were used. Eight of them were randomly chosen for the control group, while the rest were subjected to muscle contusion. Subsequently, their gastrocnemius muscles were harvested at different time points. The changes in muscle morphology were assessed by hematoxylin and eosin (HE) stain. In addition, the gene expression was analyzed by real-time polymerase chain reaction. The data showed that the expression of many genes, i.e., specific markers of immune cells and satellite cells, regulatory factors for muscle regeneration, cytokines, and chemokines, increased in the early stages of recovery, especially in the first 3 days. Furthermore, there were strict rules in the expression of these genes. However, almost all the genes returned to normal at 14 days post-injury. The sequence of immune cells invaded after muscle contusion was neutrophils, M1 macrophages and M2 macrophages. Some CC (CCL2, CCL3, and CCL4) and CXC (CXCL10) chemokines may be involved in the chemotaxis of these immune cells. HGF may be the primary factor to activate the satellite cells after muscle contusion. Moreover, 2 weeks are needed to recover when acute contusion happens as used in this study.
登录
查看更多内容
影响因子：
8
作者：
Fu, Xin;Wang, Huating;Hu, Ping
通讯作者：
Hu, Ping
影响因子：
3.7
作者：
Lesault PF;Theret M;Magnan M;Cuvellier S;Niu Y;Gherardi RK;Tremblay JP;Hittinger L;Chazaud B
通讯作者：
Chazaud B
DOI：
10.1152/ajpendo.00345.2001
发表时间：
2002-02-01
影响因子：
5.1
作者：
Farges, MC;Balcerzak, D;Baracos, VE
通讯作者：
Baracos, VE
影响因子：
64.8
作者：
Baggiolini, M
通讯作者：
Baggiolini, M
影响因子：
2.7
作者：
Cornelison, DDW;Wold, BJ
通讯作者：
Wold, BJ