Urinary phenotyping indicates weight loss-independent metabolic effects of Roux-en-Y gastric bypass in mice.
Urinary phenotyping indicates weight loss-independent metabolic effects of Roux-en-Y gastric bypass in mice.
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尿液表型分析表明 Roux-en-Y 胃绕道手术对小鼠的代谢影响与体重减轻无关
DOI：
10.1021/pr300909v
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发表时间：
2013
影响因子：
4.4
通讯作者：
Holmes E.
中科院分区：
文献类型：
--
作者：
Seyfried F;Miras AD;Cluny NL; Lannoo M;Fenske WK;Sharkey KA;Nicholson JK;Le Roux CW;Holmes E.
体重指数（BMI）高于35 kg/m2的代谢性疾病患者从Roux-en-Y胃旁路治疗（RYGB）中获益，与他们最终的BMI和体重减轻量无关。然而，RYGB诱导的与减肥无关的代谢效应仍然不太清楚。为了阐明RYGB后的代谢变化，我们获得了RYGB （n= 7）、自由喂养假手术（AL,n= 5）和体重匹配假手术（BWM,n= 5）手术小鼠的1H NMR尿液代谢谱。分析肠道形态和粪便能量含量。与sham-AL相比，RYGB小鼠的食物摄入量和体重显著降低（p= 0.001）。bwm -sham与RYGB小鼠相比，需要更少的食物来维持相同的体重（p= 0.05）。除了RYGB动物的吸收不良外，各组之间的粪便能量含量没有差异。与rygb操作的大鼠不同，rygb操作的小鼠未观察到肠道肥大。假手术组尿三羧酸循环中间体较高，提示RYGB手术后线粒体代谢改变。RYGB小鼠尿液中三甲胺、马嘌呤和葫芦巴碱水平升高表明RYGB手术引起了微生物干扰。综上所述，我们首次证明了RYGB具有特异性代谢作用，而这种作用与食物摄入和体重减轻无关。TCA循环中间体利用率的增加和肠道微生物-宿主共代谢产物的改变可能分别表明肠道中能量消耗的增加和微生物的变化。
Patients with a body mass index (BMI) above 35 kg/m2with metabolic diseases benefit from Roux-en-Y gastric bypass (RYGB) independently of their final BMI and the amount of body weight lost. However, the weight loss independent metabolic effects induced by RYGB remain less well understood. To elucidate metabolic changes after RYGB,1H NMR spectroscopy-based urine metabolic profiles from RYGB (n= 7), ad libitum-fed sham (AL,n= 5), and body-weight-matched sham (BWM,n= 5) operated mice were obtained. Gut morphometry and fecal energy content were analyzed. Food intake and body weight of RYGB mice were significantly reduced (p= 0.001) compared to sham-AL. There was a strong tendency that BWM-shams required less food to maintain the same body weight as RYGB mice (p= 0.05). No differences were found in fecal energy content between the groups, excluding malabsorption in RYGB animals. Unlike RYGB-operated rats, gut hypertrophy was not observed in RYGB-operated mice. Urinary tricarboxylic acid cycle intermediates were higher in the sham groups, suggesting altered mitochondrial metabolism after RYGB surgery. Higher urinary levels of trimethylamine, hippurate and trigonelline in RYGB mice indicate that the RYGB operation caused microbial disturbance. Taken together, we demonstrate for the first time that there are RYGB specific metabolic effects, which are independent of food intake and body weight loss. Increased utilization of TCA cycle intermediates and altered gut microbial-host co-metabolites might indicate increased energy expenditure and microbial changes in the gut, respectively.
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影响因子：
4.4
作者：
McClay, Joseph L.;Adkins, Daniel E.;van den Oord, Edwin J. C. G.
通讯作者：
van den Oord, Edwin J. C. G.
影响因子：
9
作者：
Rubino, Francesco;Kaplan, Lee M.;Cummings, David E.
通讯作者：
Cummings, David E.
影响因子：
3.7
作者：
Mutch DM;Fuhrmann JC;Rein D;Wiemer JC;Bouillot JL;Poitou C;Clément K
通讯作者：
Clément K
影响因子：
158.5
作者：
Adams, Ted D.;Gress, Richard E.;Hunt, Steven C.
通讯作者：
Hunt, Steven C.
影响因子：
14.8
作者：
Beckonert, Olaf;Keun, Hector C.;Nicholson, Jeremy K.
通讯作者：
Nicholson, Jeremy K.