Lactobacillus plantarum Exhibits Antioxidant and Cytoprotective Activities in Porcine Intestinal Epithelial Cells Exposed to Hydrogen Peroxide.
Lactobacillus plantarum Exhibits Antioxidant and Cytoprotective Activities in Porcine Intestinal Epithelial Cells Exposed to Hydrogen Peroxide.
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DOI：
10.1155/2021/8936907
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发表时间：
2021
影响因子：
--
通讯作者：
Ji H
中科院分区：
文献类型：
--
作者：
Wang J;Zhang W;Wang S;Wang Y;Chu X;Ji H
益生菌被广泛用于预防应激引起的肠道功能障碍。氧化应激在胃肠道疾病中起关键作用。益生菌可以缓解氧化应激，但其作用机制尚未阐明。建立了体外猪肠上皮细胞（IPEC-J2）氧化应激模型，以探讨植物乳杆菌ZLP 001的抗氧化作用及其可能的作用机制。将IPEC-J2细胞与L. plantarum ZLP 001处理3 h，然后用过氧化氢（H2 O2）处理4 h。L.植物ZLP 001保护IPEC-J2细胞免受H2 O2诱导的氧化损伤，如细胞活力测定所示，并显著减轻H2 O2引起的凋亡。L.与单独H2 O2处理相比，植物乳杆菌ZLP 001预处理降低了活性氧自由基的产生和细胞丙二醛浓度，并增加了线粒体膜电位，表明L. plantarum ZLP 001促进细胞中氧化还原稳态的维持。此外，L. plantarum ZLP 001通过调节某些抗氧化酶的表达和产生，从而激活抗氧化防御系统。治疗L.与单独H2 O2处理相比，植物ZLP 001导致核红细胞2相关因子2（Nrf 2）在细胞核中富集。Nrf 2基因的敲除显著减弱了L.植物乳杆菌ZLP 001对IPEC-J2细胞抗氧化应激的影响，提示Nrf 2参与了植物乳杆菌ZLP 001的抗氧化作用。plantarum ZLP 001.这些结果表明L. plantarum ZLP 001是一种有前途的益生菌，可以潜在地减轻氧化应激。
Probiotics are widely used for protection against stress-induced intestinal dysfunction. Oxidative stress plays a critical role in gastrointestinal disorders. It is established that probiotics alleviate oxidative stress; however, the mechanism of action has not been elucidated. We developed an in vitro intestinal porcine epithelial cells (IPEC-J2) model of oxidative stress to explore the antioxidant effect and potential mode of action of Lactobacillus plantarum ZLP001. The IPEC-J2 cells were preincubated with and without L. plantarum ZLP001 for 3 h and then exposed to hydrogen peroxide (H2O2) for 4 h. Pretreatment with L. plantarum ZLP001 protected IPEC-J2 cells against H2O2-induced oxidative damage as indicated by cell viability assays and significantly alleviated apoptosis elicited by H2O2. L. plantarum ZLP001 pretreatment decreased reactive oxygen species production and the cellular malondialdehyde concentration and increased the mitochondrial membrane potential compared with H2O2 treatment alone, suggesting that L. plantarum ZLP001 promotes the maintenance of redox homeostasis in the cells. Furthermore, L. plantarum ZLP001 regulated the expression and generation of some antioxidant enzymes, thereby activating the antioxidant defense system. Treatment with L. plantarum ZLP001 led to nuclear erythroid 2-related factor 2 (Nrf2) enrichment in the nucleus compared with H2O2 treatment alone. Knockdown of Nrf2 significantly weakened the alleviating effect of L. plantarum ZLP001 on antioxidant stress in IPEC-J2 cells, suggesting that Nrf2 is involved in the antioxidative effect of L. plantarum ZLP001. Collectively, these results indicate that L. plantarum ZLP001 is a promising probiotic bacterium that can potentially alleviate oxidative stress.
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影响因子：
5.8
作者：
Higuchi, Y
通讯作者：
Higuchi, Y
通过探索乳酸乳杆菌发酵液LF1的本地益生菌菌株的抗氧化潜力，改善结肠炎。
影响因子：
--
作者：
Chauhan R;Vasanthakumari AS;Panwar H;Mallapa RH;Duary RK;Batish VK;Grover S
通讯作者：
Grover S
影响因子：
6.1
作者：
Lin, MY;Yen, CL
通讯作者：
Yen, CL
影响因子：
7.3
作者：
Finamore A;Ambra R;Nobili F;Garaguso I;Raguzzini A;Serafini M
通讯作者：
Serafini M
影响因子：
4.7
作者：
Devi, Gunapathy;Harikrishnan, Ramasamy;Balasundaram, Chellam
通讯作者：
Balasundaram, Chellam