Genetic analysis and major quantitative trait locus mapping of leaf widths at different positions in multiple populations.
Genetic analysis and major quantitative trait locus mapping of leaf widths at different positions in multiple populations.
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多个群体不同位置叶宽的遗传分析及主要数量性状位点定位
DOI：
10.1371/journal.pone.0119095
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Chen Y
中科院分区：
文献类型：
--
作者：
Guo S;Ku L;Qi J;Tian Z;Han T;Zhang L;Su H;Ren Z;Chen Y
玉米叶宽是玉米的一个重要农艺性状。叶的发育依赖于细胞的增殖和扩张，这些过程相对于叶的纵轴和横轴表现出极性。然而，在玉米种子活力的遗传控制的分子机制仍然是未知的，需要更好地了解这一机制。方法/主要发现为了揭示叶宽的遗传结构，使用四个RIL群体进行了综合评价，随后进行了荟萃分析。在三种环境下的四个RIL群体中检测到46个与穗顶以上不同位置叶宽相关的QTL。QTL的个体效应占表型变异的4.33%~18.01%，其中14个QTL的效应超过10%。我们确定了三个共同的QTL与叶宽在所有的检查位置，除了一个共同的QTL与叶宽在三个位置和六个共同的QTL与叶宽在两个位置。结果表明，不同叶位的叶宽可能受一个或多个相同的QTL控制。这些性状也可能受许多不同的QTL调控。对46个初始QTL中的31个进行了Meta分析，整合到8个mQTL中，14个R2> 10%的初始QTL中有10个整合到6个mQTL中。mQTL1 - 2、mQTL3 - 1、mQTL7和mQTL8由R2> 10%的初始QTL组成，其中4~6个初始QTL与单个群体中的2~4个位点相关。因此，这四个染色体区域可能是这些性状的重要QTL的热点。因此，它们值得进一步研究，并可能是有用的标记辅助育种。
Background Leaf width is an important agricultural trait in maize. Leaf development is dependent on cell proliferation and expansion, and these processes exhibit polarity with respect to the longitudinal and transverse axes of the leaf. However, the molecular mechanism of the genetic control of seed vigor remains unknown in maize, and a better understanding of this mechanism is required. Methodology/Principal Findings To reveal the genetic architecture of leaf width, a comprehensive evaluation using four RIL populations was performed, followed by a meta-analysis. Forty-six QTLs associated with the widths of leaves at different positions above the uppermost ear were detected in the four RIL populations in three environments. The individual effects of the QTLs ranged from 4.33% to 18.01% of the observed phenotypic variation, with 14 QTLs showing effects of over 10%. We identified three common QTLs associated with leaf width at all of the examined positions, in addition to one common QTL associated with leaf width at three of the positions and six common QTLs associated with leaf width at two of the positions. The results indicate that leaf width at different leaf positions may be affected by one QTL or several of the same QTLs. Such traits may also be regulated by many different QTLs. Thirty-one of the forty-six initial QTLs were integrated into eight mQTLs through a meta-analysis, and 10 of the 14 initial QTLs presenting an R2>10% were integrated into six mQTLs. Conclusions/Significance mQTL1-2, mQTL3-1, mQTL7, and mQTL8 were composed of the initial QTLs showing an R2>10% and included four to six of the initial QTLs that were associated with two to four positions in a single population. Therefore, these four chromosome regions may be hot spots for important QTLs for these traits. Thus, they warrant further studies and may be useful for marker-assisted breeding.
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影响因子：
7.2
作者：
Kim, JH;Choi, DS;Kende, H
通讯作者：
Kende, H
影响因子：
6.8
作者：
AKAIKE, H
通讯作者：
AKAIKE, H
影响因子：
5.8
作者：
Arcade, A;Labourdette, A;Joets, J
通讯作者：
Joets, J
影响因子：
3.1
作者：
Fujino, Kenji;Matsuda, Yasuyuki;Sekiguchi, Hiroshi
通讯作者：
Sekiguchi, Hiroshi
影响因子：
2.7
作者：
Henderson, DC;Muehlbauer, GJ;Scanlon, MJ
通讯作者：
Scanlon, MJ