The role of B-cells in immunity against adult Strongyloides venezuelensis.
The role of B-cells in immunity against adult Strongyloides venezuelensis.
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DOI：
10.1186/1756-3305-6-148
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发表时间：
2013-05-24
影响因子：
3.2
通讯作者：
Ohta N
中科院分区：
文献类型：
--
作者：
El-Malky MA;Maruyama H;Al-Harthi SA;El-Beshbishi SN;Ohta N
委内瑞拉类圆线虫已被用作类圆线虫病研究的工具和模型。消除小鼠体内的委内瑞拉葡萄球菌成虫与肠道肥大细胞和嗜酸性粒细胞的增殖和激活特别相关。迄今为止，b细胞在实验动物对成年类圆线虫感染的保护机制中的作用尚未见文献报道。因此，本研究利用JH位点靶向缺失的小鼠来研究b淋巴细胞在抵抗委内瑞拉葡萄球菌感染免疫中的作用。用成年委内瑞拉葡萄球菌十二指肠内植入感染JHD基因敲除小鼠及其野生型Balb/c小鼠。计数粪卵计数、肠蠕虫恢复、粘膜肥大细胞计数和嗜酸性粒细胞计数。感染后第11天，野生型小鼠体内的寄生虫停止产卵，而JHD基因敲除小鼠体内的寄生虫继续产卵，直到观察期结束，即第107天。JHD基因敲除感染小鼠的粪便中排出的寄生虫卵数量较高，这是由于这些动物的小肠中建立了较高的蠕虫负荷。另一方面，两组小鼠的蠕虫繁殖能力相当。b细胞缺陷小鼠和野生型小鼠均表现出粘膜肥大细胞和嗜酸性粒细胞的涌入。在第11天，JHD基因敲除小鼠的绝对数量低于野生型小鼠，但没有达到显著水平。尽管招募了两种类型的细胞，但JHD敲除小鼠仍不能从感染中恢复。我们的研究结果强调了B细胞在粘膜免疫中抵抗成年委内瑞拉葡萄球菌入侵和驱逐其中的作用。因此，我们得出结论，b细胞与粘膜肥大细胞和嗜酸性粒细胞共同促进了对委内瑞拉葡萄球菌的免疫，其机制有待研究。
Strongyloides venezuelensis has been used as a tool and model for strongyloidiasis research. Elimination of S. venezuelensis adult worms from mice has been particularly associated with proliferation and activation of intestinal mast cells and eosinophils. To date, the role of B-cells in the protective mechanism against adult Strongyloides infection in experimental animals has not been reported in the literature. Therefore, the present study was carried to investigate the role of B-lymphocytes in immunity against adult S. venezuelensis infection using mice with a targeted deletion of the JH locus. JHD knockout mice with its wild-type Balb/c mice were infected by intra-duodenal implantation of adult S. venezuelensis. Fecal egg count, intestinal worm recovery, mucosal mast cells and eosinophils were counted. At day 11 post infection, parasites in wild-type mice stopped egg laying, while in JHD knockout mice parasites continued to excrete eggs until the end of the observation period, day 107. The higher number of parasite eggs expelled in the feces of JHD knockout infected mice was a consequence of higher worm burdens, which established in the small intestine of these animals. On the other hand worm fecundity was comparable in both groups of mice. Both B-cell-deficient mice and wild-type mice, showed an influx of mucosal mast cells and eosinophils. The absolute numbers in JHD knockout mice were lower than those seen in wild-type mice at day 11, but not to a level of significance. JHD knockout mice could not recover from infection despite the recruitment of both types of cells. Our findings highlight a role of B cells in mucosal immunity against invasion of adult S. venezuelensis and in its expulsion. Therefore, we conclude that B-cells together with mucosal mast cells and eosinophils, contribute to immunity against adult S. venezuelensis by mechanism(s) to be investigated.
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影响因子：
4.4
作者：
CHEN, JZ;TROUNSTINE, M;HUSZAR, D
通讯作者：
HUSZAR, D
影响因子：
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Baek, BK;Islam, MK;Kakoma, I
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作者：
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