Chemotaxis of Amoeba proteus in the developing pH gradient within a pocket-like chamber studied with the computer assisted method.
Chemotaxis of Amoeba proteus in the developing pH gradient within a pocket-like chamber studied with the computer assisted method.
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用计算机辅助方法研究了袋状室内不断发展的 pH 梯度中变形虫的趋化性。
DOI：
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发表时间：
1997
影响因子：
--
通讯作者：
Z. Madeja
中科院分区：
文献类型：
--
作者：
W. Korohoda;J. Golda;J. Sroka;A. Wojnarowicz;P. Jochym;Z. Madeja
一个新的“U”形袋状室被用来观察单个细胞的趋化反应。该方法允许监测测试物质的浓度梯度的发展和细胞运动。我们研究了变形虫的趋化反应，观察到变形虫以正向和负向发展的 [H+] 梯度向 pH 值范围为 5.75-7.75 的较低 pH 溶液移动。变形虫对 [H+] 梯度的趋化反应需要细胞外钙离子的存在。它被阻断，并且通过用细胞培养基中的镁替代钙来恢复随机运动。延时视频录制和数据处理是通过计算机辅助方法完成的。这使得可以比较各向同性和各向异性条件下细胞运动的数据呈现和分析的选定方法。确定细胞轨迹并以圆形图显示，估计细胞轨迹的长度和最终细胞位移，并计算一些表征细胞运动的参数。
A new "U" shaped, pocket-like chamber was used to observe the chemotactic responses of individual cells. This method permits monitoring of both the development of the concentration gradient of a tested substance and cell locomotion. We investigated the chemotactic responses of Amoeba proteus and observed that the amoebae moved in positively and negatively developing [H+] gradients towards the solution of lower pH in a pH range 5.75-7.75. The chemotactic response of amoebae to [H+] gradients required the presence of extracellular calcium ions. It was blocked and random locomotion was restored by the replacement of calcium with magnesium in the cell medium. Time-lapse video recording and data processing were accomplished with computer-assisted methods. This made it possible to compare selected methods of data presentation and analysis for cells locomoting in isotropic and anisotropic conditions. The cell trajectories were determined and displayed in circular diagrams, lengths of cell tracks and final cell displacements were estimated and a few parameters characterizing cell locomotion were computed.
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影响因子：
4
作者：
K. Y. Nishimura;R. Isseroff;R. Nuccitelli
通讯作者：
K. Y. Nishimura;R. Isseroff;R. Nuccitelli
影响因子：
6.5
作者：
Sheridan, DM;Isseroff, RR;Nuccitelli, R
通讯作者：
Nuccitelli, R
外部 pH 值对豚鼠基底动脉平滑肌细胞离子电流的影响。
影响因子：
20.1
作者：
West,GA;Leppla,DC;Simard,JM
通讯作者：
Simard,JM
变形虫盘基网柄菌的行为主要受天然 cAMP 波的时间动态调节。
影响因子：
--
作者：
Wessels,D;Murray,J;Soll,DR
通讯作者：
Soll,DR
空间梯度中盘基网柄菌阿米巴趋化性伪足形成的频率和方向：时间机制的进一步证据。
影响因子：
--
作者：
Varnum-Finney,BJ;Voss,E;Soll,DR
通讯作者：
Soll,DR