Gene mapping and candidate gene analysis of aberrant-floral spikelet 1 (afs1) in rice (Oryza sativa L.)
Gene mapping and candidate gene analysis of aberrant-floral spikelet 1 (afs1) in rice (Oryza sativa L.)
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水稻异常花小穗 1 (afs1) 的基因定位和候选基因分析 (Oryza sativa L.)
DOI：
10.1016/s2095-3119(19)62847-9
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发表时间：
2020-04
影响因子：
4.8
通讯作者：
Li Yunfeng
中科院分区：
文献类型：
--
作者：
Zhang Ting;You Jing;Yu Guoling;Zhang Yi;Chen Huan;Li Yidan;Ye Li;Yao Wanyue;Tu Yujie;Ling Yinghua;He Guanghua;Li Yunfeng
小穗是禾本科植物中一种独特的花序结构。然而，调节其发育的分子机制仍不清楚，因此我们表征了水稻（Oryza sativa L.）的小穗突变体，异常花小穗1（afs1），它是由甲磺酸乙酯处理西农1B衍生而来。在afs1突变体中，小穗在其他正常发育的花器官旁边发育出一个额外的外稃状器官，并且内稃不同程度地退化，有或没有正常发育的内部花器官。遗传分析表明，afs1 表型由单个隐性基因控制。 AFS1 基因定位在插入/删除 (InDel) 标记 Indel19 和简单序列重复标记 RM16893 之间，在 4 号染色体上物理距离为 128.5 kb。通过序列分析，我们鉴定了 LOC_Os04g32510/LAX2 内 5 bp 片段的删除和从 G 到 A 的颠换，这导致 afs1 突变体中翻译的提前终止。这些发现表明AFS1可能是LAX2的一个新等位基因，并通过调节与花器官发育相关的基因的表达来参与花器官的发育。上述结果为LAX2的功能提供了新的视角，LAX2也可能调控小穗的发育。
The spikelet is a unique inflorescence structure in grasses. However, the molecular mechanism that regulates its development remains unclear, and we therefore characterize a spikelet mutant of rice (Oryza sativa L.), aberrant-floral spikelet 1 (afs1), which was derived from treatment of Xinong 1B with ethyl methanesulfonate. In the afs1 mutant, the spikelet developed an additional lemma-like organ alongside the other normally developed floral organs, and the paleae were degenerated to differing degrees with or without normally developed inner floral organs. Genetic analysis revealed that the afs1 phenotype was controlled by a single recessive gene. The AFS1 gene was mapped between the insertion/deletion (InDel) marker Indel19 and the simple sequence repeat marker RM16893, with a physical distance of 128.5 kb on chromosome 4. Using sequence analysis, we identified the deletion of a 5-bp fragment and a transversion from G to A within LOC_Os04g32510/ LAX2, which caused early termination of translation in the afs1 mutant. These findings suggest that AFS1 may be a new allele of LAX2, and is involved in the development of floral organs by regulating the expression of genes related to their development. The above results provide a new view on the function of LAX2, which may also regulate the development of spikelets.
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影响因子：
44.1
作者：
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影响因子：
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发表时间：
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影响因子：
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