DNA methylation aging and transcriptomic studies in horses.
DNA methylation aging and transcriptomic studies in horses.
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DOI：
10.1038/s41467-021-27754-y
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发表时间：
2022-01-10
影响因子：
16.6
通讯作者：
Finno CJ
中科院分区：
文献类型：
--
作者：
Horvath S;Haghani A;Peng S;Hales EN;Zoller JA;Raj K;Larison B;Robeck TR;Petersen JL;Bellone RR;Finno CJ
马的胞嘧啶甲基化模式尚未得到彻底研究。在这里，我们使用定制阵列（HorvathMammalMethylChip40）在哺乳动物物种之间高度保守的位点上分析了来自42种马组织类型的n = 333个样本。利用马的血液和肝脏组织，我们开发了五种表观遗传衰老时钟：一个多组织时钟，一个血液时钟，一个肝脏时钟和两个适用于马和人类的双物种时钟。此外，利用另外三种马科动物（平原斑马、格雷维斑马和索马里驴）的血液甲基化数据，我们开发了另一种适用于所有马科动物的时钟。去势对马血液或肝脏样本的表观遗传老化率没有显著影响。来自同一组织的甲基化和RNA数据定义了马组织中甲基化和RNA表达之间的关系。我们期望多组织图谱将成为一种宝贵的资源。基因组中特定位点的甲基化水平与衰老相关。在这里，作者开发了一种人马时钟，可以帮助将抗衰老干预措施从人类翻译到马，反之亦然。
Cytosine methylation patterns have not yet been thoroughly studied in horses. Here, we profile n = 333 samples from 42 horse tissue types at loci that are highly conserved between mammalian species using a custom array (HorvathMammalMethylChip40). Using the blood and liver tissues from horses, we develop five epigenetic aging clocks: a multi-tissue clock, a blood clock, a liver clock and two dual-species clocks that apply to both horses and humans. In addition, using blood methylation data from three additional equid species (plains zebra, Grevy’s zebras and Somali asses), we develop another clock that applies across all equid species. Castration does not significantly impact the epigenetic aging rate of blood or liver samples from horses. Methylation and RNA data from the same tissues define the relationship between methylation and RNA expression across horse tissues. We expect that the multi-tissue atlas will become a valuable resource. Methylation levels of specific sites in the genome is correlated with aging. Here the authors develop a human-horse clock which could assist in translating anti-aging interventions from humans to horses and vice versa.
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影响因子：
4.6
作者：
Bernhart, Stephan H.;Kretzmer, Helene;Hoffmann, Steve
通讯作者：
Hoffmann, Steve
影响因子：
--
作者：
Harley, Eric H.;Knight, Michael H.;Gregor, Michael
通讯作者：
Gregor, Michael
DOI：
10.1097/qad.0000000000001854
发表时间：
2018-07-17
期刊：
AIDS (London, England)
影响因子：
--
作者：
Horvath S;Stein DJ;Phillips N;Heany SJ;Kobor MS;Lin DTS;Myer L;Zar HJ;Levine AJ;Hoare J
通讯作者：
Hoare J
影响因子：
5.6
作者：
Horvath S;Zoller JA;Haghani A;Jasinska AJ;Raj K;Breeze CE;Ernst J;Vaughan KL;Mattison JA
通讯作者：
Mattison JA
影响因子：
2.4
作者：
Burns EN;Bordbari MH;Mienaltowski MJ;Affolter VK;Barro MV;Gianino F;Gianino G;Giulotto E;Kalbfleisch TS;Katzman SA;Lassaline M;Leeb T;Mack M;Müller EJ;MacLeod JN;Ming-Whitfield B;Alanis CR;Raudsepp T;Scott E;Vig S;Zhou H;Petersen JL;Bellone RR;Finno CJ
通讯作者：
Finno CJ